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Invitrogen
Multispecies PARP (Cleaved) In-Cell ELISA Kit, Colorimetric
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产品规格
检测样本类型/体积
{Supernatant=10,000 cells}
手工操作时间
1 hr 20 min
总时间
24 hr
均相（无需洗涤）
No
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Blocking Buffer
20X Tris Buffered Saline
Surfact-Amps 20
Surfact-Amps X-100
HRP Conjugate
TMB Substrate
TMB Stop Solution
Janus Green Whole-Cell Stain
Elution Buffer
Thin Plate Seal Assembly
Components included only in the target-specific kits: Antibody #1 and Antibody #2
20X Tris Buffered Saline
Surfact-Amps 20
Surfact-Amps X-100
HRP Conjugate
TMB Substrate
TMB Stop Solution
Janus Green Whole-Cell Stain
Elution Buffer
Thin Plate Seal Assembly
Components included only in the target-specific kits: Antibody #1 and Antibody #2
运输条件
Wet ice
储存
2-8°C
蛋白名称
PARP1 (cleaved Asp214)
Target site-specificity
Target sub-specificity
Cleaved
蛋白家族
Other Proteins
检测试剂盒格式
In-cell ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Cleaved PARP Multispecies In-Cell ELISA Colorimetric Detection Kit is a simple method for quantifying intracellular proteins in whole cells.
原理方法
To perform the assay, cells are first plated, treated and fixed. Expression of the protein(s) of interest is monitored in wells of a microplate using target-specific primary antibodies (see the Important Product Information section for antibodies included in each kit) and a horseradish peroxidase (HRP)-conjugated detection reagent. The kit is supplied with a whole-cell stain to control for differences in cell plating, which is important when measuring relative levels of a protein with different treatments or assessing its post-translational modification (PTM) form. After staining, the results are analyzed by normalizing the absorbance (HRP activity) values to cell number, which adjusts for the cell plating differences among the wells.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
