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Invitrogen
Multispecies S6 In-Cell ELISA Kit, Near Infrared
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产品规格
检测样本类型/体积
{Supernatant=10,000 cells}
手工操作时间
1 hr 20 min
总时间
24 hr
均相（无需洗涤）
No
检测仪器
Fluorescent Microplate Reader
产品规格
96 Tests
产品成分
Blocking Buffer
20X Tris Buffered Saline
Surfact-Amps 20
Surfact-Amps X-100
DyLight® 680 Goat Anti-Mouse IgG (H+L)
DyLight 800 Goat Anti-Rabbit IgG (H+L)
Thin Plate Seal Assembly
Components included only in the target-specific kits: Antibody #1 and Antibody #2
20X Tris Buffered Saline
Surfact-Amps 20
Surfact-Amps X-100
DyLight® 680 Goat Anti-Mouse IgG (H+L)
DyLight 800 Goat Anti-Rabbit IgG (H+L)
Thin Plate Seal Assembly
Components included only in the target-specific kits: Antibody #1 and Antibody #2
运输条件
Wet ice
储存
2-8°C
蛋白名称
S6
Target site-specificity
蛋白家族
Other Proteins
检测试剂盒格式
In-cell ELISA Kit
检测抗体结合物
Unconjugated
标签或染料
DyLight™ 680 , DyLight™ 800
关于本试剂盒
The S6 Multispecies In-Cell ELISA Near Infrared Detection Kits provide a simple and convenient method for quantifying intracellular proteins in whole cells.
原理方法
This kit enables simultaneous detection of post-translational-modified protein (PTM) and the corresponding unmodified protein, or two different targets within the same well. Simultaneously detecting targets within an ELISA well eliminates variability caused by differences in cell plating. The expression levels of the protein(s) are monitored using primary antibodies specific to the targets and corresponding species-specific near-infrared Thermo Scientific DyLight-Conjugated Secondary Antibodies. Relative protein activation or inactivation is determined as a ratio of modified protein levels to the corresponding unmodified protein measured using a modification-specific antibody and an antibody to unmodified protein, respectively.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名 : Phosphoprotein NP33
