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The Mouse Osteopontin (OPN/SPP1) ELISA quantitates Ms OPN in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms OPN.
原理方法
The Mouse OPN solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases close to the integrin-binding Arg-Gly-Asp sequence modulates the function of OPN and its integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage and provide a novel substrate for plasmin and cathepsin D.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
基因别名 : 2AR, Apl-1, BNSP, Bsp, BSPI, Eta, ETA-1, OP, Opn, Opnl, Ric, Spp-1, Spp1
基因ID : (Mouse) 20750
基因符号 : Spp1
蛋白别名 : 2AR, 44 kDa bone phosphoprotein, Bone sialoprotein 1, Calcium oxalate crystal growth inhibitor protein, Early T-lymphocyte activation 1 protein, Minopontin, Osteopontin, osteopontin-like protein, Secreted phosphoprotein 1, SPP-1
UniProt ID (Mouse) P10923