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Invitrogen
Porcine EGF ELISA Kit
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产品规格
检测灵敏度
4.8 pg/mL
测定范围
4.8-2,000 pg/mL
检测样本类型/体积
{Serum=100 µL, Plasma=100 µL, Supernatant=100 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 45 min
均相（无需洗涤）
No
批间差异
12%
批内差异
10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated microwell plate
Standard
Biotinylated Detection Antibody
Streptavidin-HRP
Sample Diluent
Wash Buffer Concentrate
Assay Buffer concentrate
Stop Solution
Substrate Solution
Adhesive Plate Covers
Standard
Biotinylated Detection Antibody
Streptavidin-HRP
Sample Diluent
Wash Buffer Concentrate
Assay Buffer concentrate
Stop Solution
Substrate Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
-20°C
蛋白名称
EGF
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
Porcine EGF ELISA Kit is an enzyme-linked immunosorbent assay for quantitative detection of porcine EGF in serum, plasma, and cell culture supernatants.
靶标信息
EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : EGF, HOMG4, URG
基因ID : (Human) 1950
基因符号 : EGF
蛋白别名 : beta-urogastrone, EGF, Epidermal growth factor, H-EGF, Pro-epidermal growth factor
UniProt ID (Human) P01133
