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The Rat Albumin ELISA quantitates Albumin in serum, plasma, and other biological fluids
The Rat Albumin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Albumin is a soluble protein which constitutes approximately 50% of blood serum protein, and acts as a regulator of blood colloidal osmotic pressure and blood volume. Albumin is also an important transporter of substances within the bloodstream, such as fatty acids, thyroid hormones, metal ions and steroids.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
基因别名 : Alb, Alb1, Albza
基因ID : (Rat) 24186
基因符号 : Alb
蛋白别名 : Albumin, serum albumin
UniProt ID (Rat) P02770