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Invitrogen
Retinol Binding Protein (RBP) Urinary Competitive ELISA Kit
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产品规格
检测灵敏度
2.90 ng/mL
测定范围
3.906-1000 ng/mL
检测样本类型/体积
{Urine=25 µL}
手工操作时间
1 hr 20 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
10.1%
批内差异
5.4%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
HRP-Conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
HRP-Conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
-20°C
蛋白名称
RBP
Target site-specificity
检测试剂盒格式
Competitive ELISA
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Retinol Binding Protein (RBP Urinnary Multispecies ELISA quantitates RBP in human, rat, dog and rhesus monkey urine.
原理方法
The Retinol Binding Protein Competitive ELISA research-use-only kit is designed to quantitatively measure RBP present in urine samples. A RBP standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A RBP-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of the RBP polyclonal antibody to each well. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound RBP-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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