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Optimizing Your iFLM Correlative System Data
Date/Time: Tuesday, December 10, 2024 at 2:00 p.m. EST
Correlative light and electron microscopy is a rapidly expanding technique that researchers are using to answer complex biological questions. The Thermo Scientific iFLM Correlative System (Integrative Fluorescence Light Microscope) allows you to combine fluorescence imaging and ion milling within a single Thermo Scientific cryo-DualBeam microscope. The iFLM Correlative System is an optional, versatile, optical module for new instruments that can also be retrofitted into existing Thermo Scientific Aquilos 2 Cryo-FIBs.
Fluorescently marked cells can be located directly in the Aquilos 2 Cryo-FIB by using the iFLM Correlative System. This enables rapid identification of cell phenotypes and the subsequent selection of target sites for cryo-lamella production.
In this webinar, you’ll learn best practices in using the iFLM Correlative System to obtain optimal data. In addition, our technical experts will present a live demonstration of the Aquilos 2 Cryo-FIB to showcase the latest software features, and how to overcome common challenges during imaging and cryo-lamellae production.
About the speakers
Geoff Perumal, SEM and DualBeam Specialist, Thermo Fisher Scientific
Geoff serves as a North American SEM and DualBeam Specialist for Thermo Fisher Scientific supporting Life Sciences applications. He has extensive experience with cryo-EM and room temperature applications for SEM and DualBeam platforms. Early in his career, Geoff spent 10 years at the Albert Einstein College of Medicine, where he worked in a dual LM and EM Core Facility. He specialized in EM sample preparation and EM operation, and he developed several room temperature EM and cryo-CLEM protocols. Geoff now leverages this Core Facility experience in the industry to help researchers push the boundaries of EM techniques and applications. Geoff is always eager to speak about Thermo Fisher’s latest technologies and how to generate the highest quality EM data possible.
John Mitchels, Sr Manager, Product Management
John serves as a Sr Manager, Product Management where he primarily focuses on platforms such as Aquilos, Arctis and Hydra Bio. He is based at the factory in Brno in Czech Republic where the iFLM and FIB-SEM systems are manufactured. Prior to joining Thermo Fisher in 2015, he gained 15 years of light and electron microscopy experience by working in several different fields. At the factory in Brno, he indulges his passion for microscopy and leverage his experience developing roadmaps to advance microscopy. Even within his personal life, his wife best describes him as a ‘microscopy nut’. He is always happy to hear feedback from our microscopy users in order to advance scientific discoveries as a community.