Nucleic Acid Purification Solutions

Simplify your mRNA purification process

One challenge we can help you overcome is obtaining larger quantities of synthetic mRNA to support clinical manufacturing. POROS™ Oligo (dT)25 Affinity Resin has been designed for the purification and isolation of mRNA from in vitro transcription (IVT) manufacturing processes. This affinity chromatography resin offers high selectivity and allows for easy mRNA purification. Through the AT-base pairing mechanism, the resin effectively separates mRNA from components of the IVT process, such as enzymes, unreacted nucleotides, partial transcripts, and plasmid DNA.

Figure 1. Mechanism of action of the POROS Oligo (dT)25 affinity resin. The Poly-dT ligand allows binding with poly-A tailed mRNA molecules through AT base pairing.

Why choose the Oligo (dT)25 affinity resin?

• Easy mRNA purification from crude transcription mix
• High dynamic binding capacity and high recovery
• Ease of use – load in NaCl and elute in water or low salt buffers
• Simplified workflow helps maximize efficiency and reduce the complexity of subsequent polish steps
• Excellent scalability, enabling purification from benchtop to commercial manufacturing
• Enables a single platform purification solution to all mRNA products holding a Poly-A tail

Figure 2. Chromatogram showing efficient separation of a 2000nt mRNA from an IVT mixture at a load concentration of 2 mg/mL. Elution was performed using H2O and yielded in >95% recovery.

Resins and reagents for the purification of mRNA

Optimize your plasmid DNA purification process

The rapid growth of the gene therapy pipeline as well as genetic vaccination for various infectious diseases, requires larger scale production of plasmid DNA (pDNA) at high quality. POROS AEX resins are ideally suited for the purification of larger molecules such as plasmids and will help you overcome the common challenges of plasmid purification.

Plasmid DNA dynamic binding capacity study

During a design of experiments (DoE) study, POROS AEX resins where tested using various process conditions to determine dynamic binding capacities for plasmid DNA binding. Download the article below to learn more about the complete study.

Table 1. Dynamic Binding Capacity Determination using process conditions from DoE study. Process conditions: residence time 2.5 min, 1 mL column, 45 mS/cm conductivity

Plasmid Purification Resins

mRNA and pDNA polishing

For subsequent polishing steps after mRNA or plasmid DNA capture, we recommend our suite of differentiated novel Hydrophobic Interaction Chromatography resins.