Ion PGM™ & Ion Proton™

Rapid, affordable high-throughput sequencing solution

Ion TorrentTM is an innovative semiconductor chip-based sequencing platform. Now, InvitrogenTM project services group bring Ion PGMTM and Ion ProtonTM with rapid and accurate sequencing with to your lab.

Ion AmpliSeq™ Technology - Based on ultrahigh-multiplex PCR, target region is captured for the semiconductor high-throughput sequencing and data analysis.

Advantages:
• Only require trace amounts of sample - as little as 10 ng of input DNA, suitable for precious samples
• High efficient targeted coverage - achieve an average of 97% coverage of targeted using multiplex PCR
• Read length - available in either 200bp or 400bp read lengths

Exome sequencing

Ultra multiplex PCR exon capture technology, more than 97% coverage of the exon regions, more than 60x sequencing depths.
Providing SNP and In/Del mutation detection and analysis, gene and amino acid change annotated in dbSNP and Genome 1000.

BAC/Fosmid plasmid sequencing:

Non-model organism genome sequencing projects assisted platform

Bacterial genome de novo sequencing and re-sequencing

2011 German EHEC outbreak was first identified by the Ion PGM sequencing platform

Viral genome sequencing

Rapid discovery of new viruses

Targeting tumor gene sequencing

◆ Cancer Hotspot Panel V2: targeting 50 oncogenes and tumor suppressor genes, covering about 2,800 known mutations in exon, maximum 4000 X sequencing depth.
◆ Cancer Hotspot Panel V2: targeting 50 oncogenes and tumor suppressor genes, covering about 2,800 known mutations in exon, maximum 4000 X sequencing depth.
◆ BCRA1/2 Panel: specifically targeting breast/cervical BCRA1/2 detection, more than 1000 X sequencing depth. Angelina Jolie reducing the risk of breast cancer with genetic diagnosis, caused global hot.
◆ Colon and Lung Cancer Panel: specifically targeting 22 genes hotspots related colon cancer and lung cancer, more than 1500 X sequencing depth.

Genetic disease genetic testing

◆Inherited Disease Panel: covering 328 genes related more than 700 kinds of common Mendelian disease.
When sequencing coverage of genomic location > 20x, germline mutations > 90%
Neuromuscular disease involving 237 kinds of diseases in 88 genes
Heart disease involving 143 kinds of diseases in 61 genes
Developmental disorders involving 143 kinds of diseases in 61 genes
Metabolic diseases involving 44 kinds of diseases in 28 genes
Other typical hereditary diseases involving 75 genes of 196 diseases.

Ion AmpliSeq™ Custom Panel: design and customize the gene sequencing services according to research interests, with the flexibility to choose the sequencing depth

High-throughput sequencing and verification platforms

PCR sequencing method:

For a DNA region customers interested in, we offer primer design and synthesis, PCR amplification, preliminary results analysis of ABI3730 sequencing and experiments.

MGB probe sequencing:

Based on DNA sequence, design specific primers and probes, real-time PCR and analysis of experimental results.

SNaPshot method:

According to the DNA sequence design specific primers, single base extension by multiplex PCR, 3730 capillary electrophoresis scanning and analysis of experimental results.

Mass Spectrometry method:

According to the DNA sequence design specific primers, single base extension by multiplex PCR, mass spectrometry analysis and analysis of experimental results.

Project Service Contact

Technical Inquiry Mail Box tech-service@lifetech.com

Order Follow-up Mail Box service.cn@lifetech.com

Project Services Hotline

800-820-8982 Ext. 3; 400-820-8982 Ext. 3