Sequencing Service

Invitrogen Biotechnology Co., Ltd is equipped with internationally-advanced sequencing instruments. Our Company also possesses advanced scientific technology and vast service experience. As such we are able to satisfy customers’ demands with respect to different levels of sequencing. We are set on its mission to do even better for customers!

Our Company accepts assignments for sequencing such as bacterial strains, plasmid and PCR product. Our services are back by the following advantages:

Advanced equipment
Use of ABI 3730xl sequencing instrument complemented with BigDye Terminator kits to achieve the best sequencing effect.
State-of-the-art quality
Ensures each normal reaction reading of 800 bp
Precision efficiency
Results can be obtained within two working days upon receipt of sample, with report issued via e-mail

>> Downloand Customer Registration Form for Sequencing Service Sample

Sample requirements and primers description

Classification	Specific requirements
Unpurified PCR product	1. Fragments should be longer than 150bp； 2. Please provide 30-50μl PCR amplification products (20ng/ul, total amount of DNA>600 ng). If take 3μl sample, should be able to clearly distinguish the target band.
Purified PCR product	1. PCR products should be dissolved in ddH2O(Do not dissolved in TE solution)； 2. Concentration should be greater than 50ng/μl, the volume should be greater than 10μl (only for one reaction, for each additional reaction, another 5ul products are needed) 3. Specific band should be detected by electrophoresis.
Plasmid	1. Plasmids should be dissolved in ddH2O(Do not dissolved in TE solution)；Concentration detected by electrophoresis should be greater than 50ng/μl；the volume should be greater than 10μl (only for one reaction, for each additional reaction, another 5ul plasmids are needed)； 2. the relevant plasmid purification kits are highly recommend； 3. If possible, provide 1ml grown culture containing corresponding plasmids spare at the same time； 4. Must be marked with vector and insert fragment length, and sequencing requirements.
Liquid Cultures (Only for E. Coli.)	1. Descript the antibiotic resistance of plasmids, and we provide Ampicillin, Carbenicillin, Kanamycin, Chloramphenicol, Tetracycline and other antibiotics; other types of antibiotics required to bring their own； 2. Should be high-copy plasmids. For low-copy plasmids, please directly provide purified plasmids (concentration should be 300ng/μl, and meet other requirements for plasmid samples)； 3. Please provide >200μl overnight grown culture (12h) in sealed Eppendorf tubes stored to prevent cross-contamination or leakage；（5ml grown culture is best, so we quickly process your sample） 4. Sequencing of large numbers of samples is recommended stab culture on solid medium in disposable plastic petri dishes；
Primers	1. Concentration should be not less than 3.2pmol/µl；the volume should be greater than 10μl（more reactions, should be more primers. In common, should supply 2ul primers per reaction） 2. Random Amplified Polymorphic DNA primers（RAPD primers), degenerate primers, Oligo dT Primer and the primers Less than the length of 170bp should not be used for sequencing; 3. Generally, primer length 18-23bp is more suitable for sequencing with high specificity; 4. Provide complete sequence primer, PCR annealing temperature as possible, for reference.

Common Vector & primer

Invitrogen provides a wide range of universal primers for your selection
Download Common Vector & Primer List

Invitrogen sequencing services price list

Project	Unit	Price(Yuan)
3730 sequencing	Reaction	60
Purification of PCR product	Sample	25

Sequencing – FAQ::

>> Download Sequencing - FAQ (PDF file)

Common problematic Spectrum of DNA sequencing

>> Download Common problematic Spectrum of DNA sequencing (PDF file)