Diverse applications using smarter genomics
Genomic technology continues to evolve with advancements in next-generation sequencing (NGS), content curation and software analysis tools. The workshop will discuss a variety of applications, including high-throughput genotyping for sustainable and selective breeding in sheep and salmon as well as simplified genetic testing in cattle using NGS.
Thermo Fisher Scientific offers innovative tools and solutions to accelerate animal research. We invite you to learn how Applied Biosystems Axiom genotyping arrays, targeted genotyping by sequencing, diverse real-time PCR assays, and easy-to-use software solutions can be applied to your breeding research programs. For smarter genomics in agriculture, partnering is the answer.
Register now for our workshop, “Diverse applications using smarter genomics”, at the 2018 World Congress on Genetics Applied to Livestock Production (WCGALP) by filling out the short form.
Date: Tuesday, February 13, 2018
Time: 7:00 a.m.–8:30 a.m.
Location: Aotea Centre, Auckland, New Zealand
Breakfast will be provided
Welcome and Introduction
Agrigenomics Product Management Leader
Thermo Fisher Scientific
Low Cost and High Information Content—the Crux to Achieving High Penetrance of Genomic Technologies in the Sheep Sector
Quantitative Genetics Research Officer
Carefully selected genotype panels, as well as providing sufficient information for use in genomic evaluations, can provide information for accurate parentage assignment, estimation of inter-animal co-ancestry (and its use in subsequent mating advice), monitoring of variants conferring major effects or congenital effects, and karyotyping. Consistency in genotype calls and file formats across panel vendors and genotyping service providers are vital in achieving low cost genotyping through intense competition.
Analysis of single nucleotide polymorphism (SNP) data from 84 animals genotyped on both Axiom and Illumina platforms revealed excellent concordance, especially if limited to high quality SNPs; the genotype concordance between vendors for the high quality SNPs was 99.5%. These results therefore imply that the platform is irrelevant when it comes to merging of genotypes; genotype results from Axiom can be outputted in several different formats, including that used by Illumina. Subsequently an Axiom genotype panel was developed to provide high quality genotype calls at a low cost, to facilitate the generation of considerable information for use by sheep producers and the sheep sector as a whole.
Customizing Genomic Profiles for Dairy and Beef Cattle Using Targeted GBS
Aaron Larsen, PhD
Chief Science Officer
United Bio Research, Inc.
Utilizing genomic information to increase profitability and improve production in livestock has become an essential component of modern breeding strategies. With rapidly changing genomic technology accelerating the rate of discovery of new genetic markers, it is important that genetic laboratories adapt quickly and provide cost-effective solutions for producers to take advantage of this information. The targeted genotyping-by-sequencing (GBS) approach using the Applied Biosystems AgriSeq workflow streamlines the process of developing custom assays and provides the flexibility to quickly modify or combine existing assays.
Here, we present an example of using the AgriSeq workflow to develop genetic assays for both beef and dairy cattle. We’ve combined hundreds of genetic markers associated with production traits, genetic mutations associated with disease, and a parentage panel based on the 200 ISAG Bovine SNPs into a single test. In addition, we’ve used this technology to simultaneously detect larger genomic variations, including Y-chromosome detection used for gender identification and the presence of large duplications that cannot be detected by a single variant.
Salmon Breeding Transformed: Marine Harvest Enters the Genomic Era with Axiom Genotyping Technology
Matt Baranski, PhD
Marine Harvest Norway
Marine Harvest ASA is one of the largest seafood companies in the world and the world’s largest producer of Atlantic salmon. In 2017, Marine Harvest’s selective breeding program transitioned to a full implementation of genomics using Axiom genotyping technology. Over 60,000 samples were genotyped, and through a combination of genome-wide association studies and genomic selection, the breeding program has dramatically increased the accuracy of selection for traits including growth rate, disease resistance and product quality. Efficient pipelines have been established for logistics, data handling and analysis, and the breeding program now represents one of the most advanced for any aquaculture species.