Solid-phase extraction is a technique used to purify and/or concentrate certain compounds in a liquid mixture prior to chromatographic analysis. Compounds in the aqueous mixture are separated based on physical and chemical properties that give them more or less affinity for a solid phase. The technique can be used successfully for urine, blood, water, beverages, soil, and animal tissue. During the process, one part of the sample binds to the stationary phase while the other passes through. In some cases, the compound of interest has a low affinity for the solid phase and passes through while other compounds are retained. In those cases, the liquid is collected for analysis. In other cases, the compound of interest has a high affinity for the solid phase, while other compounds pass through. In that case, the liquid that flows through is discarded and a different eluent is used to elute the compound from the stationary phase. It is then collected for further analysis. In either case, the result is a solution enriched for the analyte of interest, making that analyte stand out from background noise on a chromatogram.

Automated solid-phase extraction is ideal for laboratories looking to automate large-volume water extractions. Typical applications include PAH, PCB, PCDD, PCDF, PFAS, pesticides, herbicides, flame retardants, semivolatiles, nitrosamines, and steroids. Applications for the Thermo Scientific Dionex AutoTrace 280 SPE system include many US EPA methods and a variety of other SPE sample preparation cleanup methods including:

  • US EPA Method 525
  • US EPA Method 537
  • US EPA Method 549.2
  • US EPA Method 508 & 608
  • US EPA Method 625
  • Total petroleum hydrocarbons
  • Acid herbicides
  • Triclosan
  • Nitrosamines (US EPA Method 521)
  • Steroids and phytoestrogens
  • Endocrine disruptors (US EPA Method 1694)
  • Personal care products

Thermo Scientific Dionex AutoTrace 280 Solid-Phase Extraction Instrument

The Dionex AutoTrace 280 SPE Instrument automates conditioning, loading, rinsing, and elution to reduce solvent consumption and improve recovery and reproducibility. It can process up to six samples in 2–3 hours with just 15 minutes of hands-on time. 

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Thermo Scientific Dionex AutoTrace 280 SPE PFAS

Designed specifically for preparation of samples for PFAS analysis, the Dionex AutoTrace 280 PFAS reduces the risk of background contamination using non-fluoropolymer-based tubing, valves, and filters. It ensures compliance with US EPA 537, 537.1 and 533.
 

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Improve your SPE process for preparing PFAS samples

The new Dionex AutoTrace 280 SPE PFAS automates SPE and reduces background contamination.

Fundamentals of solid phase extraction

Hear about the theory and concept of solid phase extraction, sample preparation options and a step-by-step guide to troubleshooting.

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从大体积水性基质中自动分离痕量有机物

采用 Thermo Scientific™ Dionex™ AutoTrace™ 280 自动固相萃取(SPE)系统从大体积水性基质分析痕量有机物。该自动系统可捕获和浓缩待测分析物,所以一次运行可在 2-3 小时内处理 6 个样品,操作时间仅 15 分钟。典型应用包括 PAH、PCB、PCDD、PCDF、农药、除草剂、阻燃剂、半挥发性物质、亚硝胺和类固醇。

特定的加速溶剂萃取类别

该系统可实现达 6 种样品的自动 SPE——来自萃取柱或萃取盘调节、样品加载、冲洗和洗脱步骤、清洗和洗脱。

Dionex SolEx SPE 萃取柱有多种化学键合相和尺寸可供选择,可捕获和浓缩大体积水性基质中的待测分析物。

配件包括试剂瓶、试管、支架以及与 Dionex Auto Trace 280 配用的维修工具包。

Dionex AutoTrace 的常见应用

Dionex AutoTrace 280 自动固相萃取仪非常适合寻求自动化大体积水萃取的实验室。典型应用包括 PAH、PCB、PCDD、PCDF、农药、除草剂、阻燃剂、半挥发性物质、亚硝胺和类固醇。Dionex AutoTrace 系统应用含多个美国 EPA 方法和各种其它 SPE 样品制备净化方法,包括:

  • 美国 EPA 方法 525
  • 美国. EPA 方法 549.2
  • 美国 EPA 方法 508 & 608
  • 美国 EPA 方法 625
  • 美国 EPA 方法 1664
  • 总石油烃
  • 酸性除草剂
  • 三氯生
  • 亚硝胺(EPA 方法 521)
  • 类固醇和植物雌激素
  • 内分泌干扰物(EPA 方法 1694)
  • 个人护理用品

Dionex SolEx SPE Cartridge Materials

Cartridge Type

Format

Functional Group

Example Application

HRPHS3mL or 6mL barrelsDivinylbenzene (DVB / polyvinylpyrrolidone (PVP, high)General reversed phase, polyphenolics, azo compounds
SAX3mL or 6mL barrelsDVB-quaternary ammoniumIonizable (weak) anions, arboxylic acids
SCX3mL or 6mL barrelsDVB-sulfonateIonizable (weak) cations, amines
WAX3mL or 6mL barrelsDVB-aminePermanent anions, inorganic and organic, sulfonates, sulfates
WCX3mL or 6mL barrelsDVB-carboxylatePermanent cations, inorganic and organic, quaternary ammonium
HRPOn-line cartridge and RSLC columnDVB-PVP (low)General neutral organics
C8, C181mL, 3mL or 6mL barrelsBonded silicaPesticides, herbicides, hydrocarbons
Phthalate-free C8, C186mL barrelsBonded silicaTrace analysis
Silica6mL barrelsAcid-washed unbonded silicaPolar compounds
Activated Carbon6mL barrelsCharcoalNitrosamines, 1,4-dioxane
Graphitized Carbon6mL barrelsGraphitized carbon particlesHerbicides

Dionex AutoTrace 的常见应用

Dionex AutoTrace 280 自动固相萃取仪非常适合寻求自动化大体积水萃取的实验室。典型应用包括 PAH、PCB、PCDD、PCDF、农药、除草剂、阻燃剂、半挥发性物质、亚硝胺和类固醇。Dionex AutoTrace 系统应用含多个美国 EPA 方法和各种其它 SPE 样品制备净化方法,包括:

  • 美国 EPA 方法 525
  • 美国. EPA 方法 549.2
  • 美国 EPA 方法 508 & 608
  • 美国 EPA 方法 625
  • 美国 EPA 方法 1664
  • 总石油烃
  • 酸性除草剂
  • 三氯生
  • 亚硝胺(EPA 方法 521)
  • 类固醇和植物雌激素
  • 内分泌干扰物(EPA 方法 1694)
  • 个人护理用品

Dionex SolEx SPE Cartridge Materials

Cartridge Type

Format

Functional Group

Example Application

HRPHS3mL or 6mL barrelsDivinylbenzene (DVB / polyvinylpyrrolidone (PVP, high)General reversed phase, polyphenolics, azo compounds
SAX3mL or 6mL barrelsDVB-quaternary ammoniumIonizable (weak) anions, arboxylic acids
SCX3mL or 6mL barrelsDVB-sulfonateIonizable (weak) cations, amines
WAX3mL or 6mL barrelsDVB-aminePermanent anions, inorganic and organic, sulfonates, sulfates
WCX3mL or 6mL barrelsDVB-carboxylatePermanent cations, inorganic and organic, quaternary ammonium
HRPOn-line cartridge and RSLC columnDVB-PVP (low)General neutral organics
C8, C181mL, 3mL or 6mL barrelsBonded silicaPesticides, herbicides, hydrocarbons
Phthalate-free C8, C186mL barrelsBonded silicaTrace analysis
Silica6mL barrelsAcid-washed unbonded silicaPolar compounds
Activated Carbon6mL barrelsCharcoalNitrosamines, 1,4-dioxane
Graphitized Carbon6mL barrelsGraphitized carbon particlesHerbicides