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MDi technology
Thermo Fisher Scientific MDi technology integrates advanced monodisperse particle platforms with bio-inert hardware to deliver durable, reproducible chromatographic performance. We incorporate MDi technology in Thermo Scientific SurePac Bio SEC MDi columns, SurePac Oligo RP MDi columns, and SurePac Protein RP MDi columns, as well as in Thermo Scientific ProPac 3R SAX and SCX columns.
SurePac Oligo RP MDi columns — 2.5 µm supermacroporous polymeric particles enable reproducible, high-efficiency reversed-phase separations for therapeutic oligonucleotides and nucleic acids of any size.
SurePac Protein RP MDi columns — 2.5 µm monodisperse polymeric particles enable reproducible, high-efficiency reversed-phase separations for monoclonal antibodies, antibody–drug conjugates, and other therapeutic proteins. as well as in Thermo Scientific ProPac 3R SAX and SCX columns.
SurePac Bio 550 SEC MDi columns - the 3 µm monodisperse silica provides superior resolution of adeno associated virus (AAV) and aggregates in cell and gene therapy applications.
ProPac 3R SAX and SCX columns - our proprietary 3 µm monodisperse polymeric technology which supports the delivery of reproducible, high resolution and robust strong anion and strong cation exchange chromatography separations for proteins, monoclonal antibodies, AAV, and other emerging modalities.
Left - 3 µm MDi polymeric monodisperse technology. Right – polydisperse non-porous media
Monodisperse particles
The advantages of monodisperse particles in chromatography column technology
In chromatography column technology, particle size distribution directly impacts packing uniformity and overall method performance. MDi technology uses tightly controlled monodisperse particles—available in porous or non-porous formats depending on the application—to deliver consistent, reliable chromatographic results.
- Reproducibility: The narrow particle size distribution of MDi particles promotes homogeneous packing and consistent bed structure. This improves column-to-column and batch-to-batch reproducibility, reducing variability and supporting robust, easy-to-validate analytical methods.
- Packing uniformity: Uniform particle size reduces fines and limits heterogeneity in the packed bed. This leads to predictable chromatographic behavior and stable performance over time, improving method reliability across instruments and laboratories.
- Performance flexibility: MDi technology uses both porous and non-porous particle architectures, matched to column chemistry and application. Optimized particle sizes deliver application-specific performance without compromising reproducibility.
Inert column hardware
Inert hardware refers to the use of materials that eliminate interactions with the sample, ensuring sample integrity and recovery. This type of hardware is particularly advantageous in biopharmaceutical analysis, where the accuracy and reliability of results are crucial. By reducing unwanted interactions, inert hardware helps to maintain sample integrity, prevent degradation or adsorption, and promote injection-to-injection reproducibility.
- Maintains the integrity of the target molecule by minimizing interactions between the column hardware and the sample.
- Promotes reproducibility and consistency in analysis by eliminating or reducing interactions between the hardware and the sample.
- Offers excellent chemical compatibility, allowing for a wide range of solvents and mobile phases to be used without compromising the integrity of the column.
- Improves reproducibility by maintaining consistent separation performance and sample recovery across multiple runs.
Inert column hardware is essential—but true performance gains come from a fully bio-inert LC workflow. Explore our bio-inert LC solutions to see how the entire flow path is optimized to protect your analytes and ensure reliable results.
SurePac Oligo RP MDi columns
SurePac Oligo RP MDi columns combine monodisperse polymeric particle technology with bio-inert hardware to deliver reproducible, high-efficiency reversed-phase separations for therapeutic oligonucleotides and nucleic acids of any size. Designed to withstand elevated pH and temperature conditions commonly used in LC–MS and LC–UV workflows, these columns provide sustained resolution of truncation impurities, preserved peak shape, and minimal carry-over.
SurePac Oligo RP MDi columns
SurePac Protein RP MDi columns
SurePac Protein RP MDi columns leverage monodisperse polymeric particle technology to deliver robust, high-resolution reversed-phase separations for proteins including monoclonal antibodies and antibody–drug conjugates. Engineered for superior thermal and chemical resistance, these columns maintain performance under the elevated temperatures typically used to optimize peak shape in LC–MS workflows.
The durable polymeric packing ensures consistent retention behavior, sustained efficiency, and reliable column lifetime across intact and subunit protein analysis, supporting both advanced characterization and routine QC applications.
SurePac Protein RP MDi columns
ProPac 3R columns
ProPac 3R SCX and SAX 3 μm columns provide the reproducibility, resolution, and robustness needed for protein characterization. These columns are ideal for charge variant analysis and recommended for use with therapeutic proteins, monoclonal antibodies (mAbs), antibody drug conjugates (ADCs), and biosimilars.
Designed with bioinert materials to reduce secondary interactions, our ProPac 3R SCX and SAX columns deliver the performance you need over a broad range of pH's, temperatures, and mobile phase compositions with excellent recovery and low carryover.
ProPac 3R SCX columns
ProPac 3R SAX columns
SurePac Bio 550 SEC MDi columns
SurePac Bio 550 SEC MDi columns are exclusively designed to deliver robust and reproducible performance at high resolution and throughput to accelerate your therapeutics analysis process.
Designed with bioinert materials to reduce secondary interactions, SurePac Bio 550 SEC MDi columns are ideal for AAV gene therapeutics analysis and offers excellent selectivity due to the dedicated smaller particle (3 µm) size combined with wide-pore (550Å) for separating monomeric AAVs from aggregates based on their size differences.
SurePac Bio 550 SEC MDi columns
SurePac Bio 550 SEC MDi guard columns
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For Research Use Only. Not for use in diagnostic procedures.