Innovative products for the molecular cloning workflow

The molecular cloning workflow requires multiple steps to obtain high-quality cloned DNA for your downstream applications. Help advance your molecular cloning workflow at every step and accelerate construction of recombinant clones.

Our wide range of cloning solutions address needs from routine to specialty applications, helping you find optimal reagents to achieve fast results with greater confidence.

Molecular cloning workflow

Click on each step to explore the molecular cloning workflow and select products for your downstream applications that fit your research.

Find products for your cloning experiments by exploring 5 steps of the cloning workflow

Select from a variety of reagents and instruments to generate your fragment of interest from DNA, RNA, or synthetic fragments.

PCR Enzymes & Reagents

PCR enzymes for genotyping and routine PCR. Characterized by high fidelity and high specificity, these enzymes are suitable for amplifying long fragments and GC-rich templates.

Reverse Transcription

Reverse transcriptase synthesizes cDNA from single-stranded RNA and serves as a template for PCR amplification. Explore our selection tool to find the right reverse transcriptase for your experiment.

Oligonucleotides

Custom DNA Oligos Synthetic Services provide oligos in a variety of scales, formats, modifications, and purifications. Use our easy ordering portal for oligos that meet your cloning needs.

Thermal Cyclers

Applied Biosystems thermal cyclers are designed for reliability and accuracy that helps generate consistent and reproducible PCR results. Use with validated PCR and qPCR plastics for optimal fit and performance.

PCR Plastics

Explore the online plastics selection tool to find the right fit and compatible plastics for your PCR or qPCR instruments. Validated for use with Applied Biosystems thermal cyclers.

Nucleotides

dNTPs, NTPs, and modified nucleotides are synthesized from high-quality raw materials in state-of-the-art production facilities and are available in a variety of formats, formulations, and volumes for convenience and flexibility.

Strings DNA Fragments

GeneArt Strings DNA Fragments are custom-made, uncloned, double-stranded linear DNA fragments assembled from synthetic oligonucleotides. Excellent for multi-fragment assembly with Gibson Assembly cloning kits.

PureLink DNA Purification Kits

PureLink DNA purification kits are an easy, fast, and safe method for isolating DNA fragments that yield excellent results. Provides purified DNA fragments ready for your cloning applications.

GeneJET DNA Purification Solutions

GeneJET nucleic acid purification kits rely on convenient spin column-based technology that offers reliable and affordable purification of DNA and RNA from different sources to meet your cloning needs.

Up next: Step 2, Cloning ›

Discover reagents and techniques across the molecular cloning workflow, with solutions ranging from restriction enzymes to gene synthesis, designed for speed and simplicity to help you obtain high-quality cloned DNA for your next discovery.

FastDigest Restriction Enzymes

FastDigest restriction enzymes are an advanced line of 176 restriction enzymes in a complete, one-buffer system for simplified cloning. Performs complete digestion in 5–15 minutes and is compatible with downstream applications.

Modifying Enzymes

Modifying enzymes offer reliable, high-quality, high-purity reagents, including DNA and RNA polymerases, phosphatases, kinases, and nucleases for an efficient cloning workflow.

DNA Vectors

A wide selection of vectors available in kits and as a stand-alone product to transfer foreign genetic material into a host cell for your downstream applications.

TOPO PCR Cloning

TOPO PCR kits enable cloning in 5 minutes with three easy steps, including Blunt-End, TA and Long Fragment cloning kits. Explore our selection tool to find the right kits for your cloning experiments.

Gateway Cloning Technology

Gateway recombination cloning can help empower gene shuttling, enabling you to access virtually any expression system, including E. coli, mammalian, and insect, in just a few simple steps.

Gibson Assembly® Cloning

Gibson Assembly® cloning kits are designed for efficient and seamless assembly of multiple DNA fragments of varying length into any vector. Excellent for multi-fragment assembly with GeneArt Strings DNA Fragments.

GeneArt Seamless Cloning

GeneArt Seamless Cloning kits enable the simultaneous and directional assembly of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector for diverse applications.