pSilencer 4.1-CMV neo Vector Map

pSilencer 4.1-CMV neo Vector Map

This map and the annotated sequence below portrays the circular negative control vector provided with the kit. It includes the negative control siRNA template (55 base pairs) between the BamH I and Hind III sites.

pSilencer 4.1-CMV neo Annotated Promoter/Cloning Site Region

We recommend the following primers for sequencing:

Forward: 5'-AGGCGATTAAGTTGGGTA-3'

Reverse: 5'-CGGTAGGCGTGTACGGTG-3'




pSilencer 4.1-CMV neo Restriction Sites

Enzymes with single Restriction Sites

Site Enzyme
1110Acc65I
1574AflIII
2467AhdI
539AloI
507AloI`
1990AlwNI
4431AvaI
4452AvrII
515BanHI
3885BanII
2972BcgI
3006BcgI`
4401BclI
1104BglII
405BmtI
4387BsaBI
2528BsaI
1449BsaXI
1419BsaXI`
45BsmBI
3326BsmI
3845BssHII
4933EcoO109I
1118EcoRI
462HindIII
1114KpnI
4164MscI
401NheI
406NotI
3562NruI
1574PciI
3385PsiI
4197PstI
3727RsrII
2947ScaI
4684SexAI
4433SmaI
763SnaBI
4457StuI
4127Tth111I
4431XmaI
3066XmnI

Non-Cutting Enzymes:

Enzyme
AarI
AccI
AfeI
AflII
AgeI
AleI
ApaI
AscI
AsiSI
BaeI
BaeI'
BbsI
BbvCI
BlpI
BmgBI
Bpu10I
BsgI
BsiWI
BspEI
BsrGI
BstBI
BstEII
BstXI
BstZ17I
Bsu36I
BtrI
ClaI
DraIII
EcoICRI
EcoNI
EcoRV
FseI
FspAI
HincII
HpaI
MfeI
MluI
PacI
PflMI
PmeI
PmlI
PpuMI
PshAI
PspOMI
PsrI
PsrI'
SacI
SacII
SalI
SanDI
SbfI
SfiI
SgfI
SgrAI
SpeI
SrfI
SwaI
TaqII'
XbaI
XcmI
XhoI