Search
Bring the Power of TaqMan Assays to Your dPCR Platform
Use Applied Biosystems TaqMan Gene Expression Assays for dPCR with confidence. TaqMan Assays comprise an extensive collection of predesigned research assays covering a broad range of gene targets. Assays are available with multiple fluorophore options, enabling flexible dye assignment to fit instrument capabilities and multiplex design requirements. Since the same verified assay designs can be used in both qPCR and dPCR workflows, researchers can maintain continuity across platforms while adapting to evolving experimental needs.
TaqMan Gene Expression Assay performance on a nanoplate-based platform
Serially diluted cDNA showed consistent, fluorophore-independent quantification across platforms.
Figure 1. Consistent RPLP0 quantification across fluorophores and platforms. Quantities were calculated by normalizing each dilution to the mean concentration of the undiluted sample for each dye. Two-fold serial dilutions were prepared from the highest input sample. The x-axis represents expected value based on dilution factor, and the y-axis shows mean normalized values. Error bars represent standard deviation of six replicates for dilutions 1, 0.5, 0.25, and 0.125 and eight replicates for dilution 0.0625. (A) Representative RPLP0 quantification for FAM, VIC, and cyanine 5 on the MAP-based platform. (B) Representative RPLP0 quantification for FAM, VIC, and cyanine 5 on the nanoplate-based platform.
CD274 expression increased 10% incrementally, matching small RNA input changes.
Figure 2. Relative quantification of CD274 in contrived lung cancer samples on two dPCR platforms. Samples containing 10% incremental increases in CD274 relative to the control sample were tested on both the MAP-based and nanoplate-based dPCR platforms. Relative quantification (RQ) values were calculated by normalizing CD274 concentration to CIAO1 within each reaction, then expressing that value relative to the reference control (Control). Bars represent mean RQ values, and error bars indicate the standard deviation for 12 replicates.
Together, these findings demonstrate that TaqMan gene expression assays provide a flexible, instrument-agnostic solution for dPCR gene expression workflows. With an extensive portfolio of predesigned TaqMan assays, multiplexing support tools, and access to specialty oligo services for alternative dye configurations, researchers can design and implement multiplex gene expression panels tailored to their specific instrument requirements and experimental needs.
TaqMan Gene Expression Assay performance on a droplet-based platform
Consistent performance across fluorophores
Figure 3. Quantities were calculated by normalizing each dilution to the mean concentration of the undiluted sample for each dye. Two-fold serial dilutions were prepared from the highest input sample. The x-axis represents expected value based on dilution factor, and the y-axis shows mean normalized values. Error bars represent standard deviation of four replicates per dilution.
CD274 expression demonstrated consistency even with small-fold changes
Figure 4. Relative quantification of CD274 in contrived lung cancer samples on two dPCR platforms. Samples containing 10% incremental increases in CD274 relative to the control sample were tested on both the MAP-based and droplet-based dPCR platforms. Relative quantification (RQ) values were calculated by normalizing CD274 concentration to CIAO1 within each reaction, then expressing the normalized value relative to the reference control (Control). Bars represent mean RQ values, and error bars indicate the standard deviation for four replicates.
dPCR precision and speed to put you above the curve
The Applied Biosystems QuantStudio Absolute Q Digital PCR System is a plate-based digital PCR platform powered by proprietary microfluidic array plate (MAP) enabling consistent delivery of >20,000 microchambers and less than 5% dead volume.
Related pages
For Research Use Only. Not for use in diagnostic procedures.