EXPRESS One-Step Superscript™ qRT-PCR 试剂盒,通用型
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Invitrogen™

EXPRESS One-Step Superscript™ qRT-PCR 试剂盒,通用型

EXPRESS One-Step SuperScript qRT-PCR 试剂盒采用经过验证的 Invitrogen 酶,专用于在高通量、Fast 型循环仪上为探针式 qPCR了解更多信息
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货号反应次数
1178101K2500 次反应
11781200500 次反应
货号 1178101K
价格(CNY)
69,107.00
Each
添加至购物车
反应次数:
2500 次反应
价格(CNY)
69,107.00
Each
添加至购物车
EXPRESS One-Step SuperScript qRT-PCR 试剂盒采用经过验证的 Invitrogen 酶,专用于在高通量、Fast 型循环仪上为探针式 qPCR 实验提供出色的结果。这种快速的一步法预混液设计为可配合各种仪器的默认方案使用,包括 Applied Biosystems 和非 Applied Biosystems(参见通用版本) qPCR 平台。

EXPRESS One-Step Superscript qRT-PCR 试剂盒的特点包括:
快速激活、抗体介导的 Platinum Taq DNA 聚合酶 – 完全、快速激活,可提供更佳的灵敏度和重现性
一步法 SuperScript III 逆转录酶 – 具有独特的配方,可确保更高的得率和更低的 PCR 抑制率
灵活的形式,包含单独或预混的 ROX – 旨在配合广泛的高通量快速仪器(例如 AB 7500 Fast、AB 7900 HT Fast、AB StepOne、Roche LightCycler™ 480、Corbett Rotor-Gene™)上默认的仪器方案一起使用
含有不耐热 UDG – 可显著降低残留污染风险,并且在从室温到约 40°C 温度范围内均具有出色的活性。由于 SuperScript III 的热稳定性,cDNA 合成步骤在 50°C 条件下运行,在该温度下,不耐热的 UDG 被完全且不可逆地灭活

EXPRESS 试剂盒可配合快速仪器上生产商的默认方案使用,并采用便利、稳定的 SuperMix 形式,具有极佳的便利性。这些试剂盒提供预混 ROX 或单独的 ROX 管,以满足您的仪器需求,同时提供 SYBR Green 化学选项:
EXPRESS One-Step Superscript qRT-PCR 试剂盒,通用型(该页)
EXPRESS One-Step Superscript qRT-PCR 试剂盒,包含预混 ROX
EXPRESS One-Step SYBR GreenER 试剂盒,包含预混 ROX
EXPRESS One-Step SYBR GreenER 试剂盒,通用型
仅供科研使用。不可用于诊断程序。
规格
适用于(设备)7500 Fast 系统,7500 Fast 系统,Applied Biosystems StepOnePlus™ 快速实时荧光定量 PCR 系统,BioRad CFX96™,StepOnePlus™,Fast 模式,StepOnePlus™,标准模式,Stratagene Mx3000P,Stratagene Mx3005P,Stratagene Mx4000,BioRad MyiQ,Corbett RotorGene,Cepheid SmartCycler,MJ Opticon,MJ Chromo4,BioRad iCycler iQ
产品规格管装
反应次数2500 次反应
聚合酶Taq DNA 聚合酶
产品线SuperScript™
产品类型qRT-PCR 试剂盒
数量2,500 reactions
样品类型RNA
足够用于2500 次反应
检测方法引物-探针
PCR 方法一步法 RT-qPCR
反应速度快速,标准
Unit SizeEach
内容与储存
EXPRESS 一步法 SuperScript™ qRT-PCR 通用试剂盒含有 3 个模块:2X EXPRESS SuperScript™ 混合物 (5 × 1 mL)、Express qPCR SuperMix Universal (5 × 5 mL) 和 ROX 参比染料 (5 × 500 µL)。

在 -20°C 下长期储存。解冻后,EXPRESS qPCR SuperMix 可在 4–8°C 下储存长达 1 个月。

常见问题解答 (FAQ)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.