Custom Applied Biosystems™ TaqMan MGB (minor groove binder) probes incorporate a 5' reporter and a 3' nonfluorescent quencher (NFQ). The NFQ offers the advantage of lower background signal, which results in better precision in quantitation. Custom TaqMan MGB probes are HPLC-purified and available with Applied Biosystems™ FAM™, VIC™, TET™, and NED™ reporter dyes.
|Catalog number||Unit size*||Reporter dye||Quencher||Format|
|4316034||6,000 pmol||FAM, VIC, TET, or NED||MGB NFQ||1 tube||Order now|
|4316033||20,000 pmol||FAM, VIC, TET, or NED||MGB NFQ||1 tube||Order now|
|4316032||50,000 pmol||FAM, VIC, TET, or NED||MGB NFQ||1 tube||Order now|
*Minimum amount delivered
Shorter, more specific probes
TaqMan MGB probes include a minor groove binder (MGB) moiety at the 3’ end that increases the melting temperature (Tm) of the probe and stabilizes probe–target hybrids. This means that TaqMan MGB probes can be significantly shorter than traditional probes, providing better sequence discrimination and flexibility to accommodate more targets.
Nonfluorescent quencher (NFQ) maximizes sensitivity
TaqMan MGB probes incorporate an NFQ to absorb (quench) signal from the fluorescent dye label at the other end of the probe. The properties of the NFQ combined with the short length of the MGB probe result in lower background signal than with non-MGB NFQ probes. Lower background means increased sensitivity and precision in your data.
Liquid 1X TE
|5’ reporter dye options||FAM, VIC, TET, NED|
|Shelf life||12 months from manufacturing date|
|Green features||Less waste, sustainable packaging|
|Shipping condition||Room temperature|
Figure 1. TaqMan probes provide better sensitivity and precision.
Comparison of two 5´ nuclease PCR assays for 18S rRNA. Ten-fold dilutions of Universal Human Reference RNA (10–10–5 ng) were prepared and analyzed in 11 replicate real-time PCR reactions using either the TaqMan Gene Expression Assay (FAM dye–labeled, with NFQ) or the non-MGB assay (FAM dye–labeled, with BHQ). Real-time PCR was run according to the respective manufacturers’ recommended conditions. Across a 6-log range of input template, the TaqMan Assay displayed earlier Ct values and better reproducibility across all data points. In addition, the TaqMan Assay had higher signal and lower background, resulting in better sensitivity and higher precision.