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免疫细胞成像ICC/IF实验流程指南

免疫细胞成像,常常把ICC/IF(免疫细胞化学Immunocytochemistry /免疫荧光Immunofluorescence)放在一起使用,是非常常用的实验技术,通过使用抗原抗体特异性结合反应,来分析靶抗原的相对丰度、构象和亚细胞定位等。我们可提供备受全球科学家认可和经过长期验证的Molecular Probes™荧光标记试剂和Invitrogen™抗体,帮您获得可靠的高品质出版级细胞图像。

ICC/IF实验流程指南手册

本手册按照标准ICC/IF 实验方案的每一步,介绍了实验描述、贴心小建议、产品推荐、选择指南等,帮您获取最优质的出版级细胞图像。
 
 

下载ICC/IF实验流程手册

 

荧光光谱查看器

特别实用的小工具,可绘制和比较不同染料的荧光光谱,检查多种荧光基团的光谱兼容性,并以清晰的打印格式将配置参数下载或发email。
 

免费使用光谱查看器

 

Molecular Probes 荧光大课堂

由经验丰富的资深研发科学家开发,希望为使用荧光试剂和抗体的科学家提供有关荧光的基础知识、技术信息及实验设计和实验方案、实用技巧等,荧光基础、显微镜成像、流式细胞术三个模块。

马上在线学习

 

产品

  • 1细胞准备
  • 2固化、透化、封闭
  • 3免疫标记
  • 4多色检测&复染
  • 5信号优化

1细胞准备

健康的细胞是后续实验的基础,至关重要。实验开始前,需根据研究目的对细胞进行修饰或加药刺激等处理,并制作细胞爬片/ 涂片,培养至均匀合适的融合度。

Invitrogen可提供Nunc™ Lab-Tek 腔室载玻片及其他相关产品。

玻片、盖玻片、多孔垫片等辅助产品促销代码P2518300

货号产品名称规格目录价数量
C14784推荐Coverslip Mini-Rack, for 8 coverslips1 Each1,782
C37010推荐Coverglass Removal Tool1 each230
C37000CultureWell Chambered Coverglass for Cell Culture, 16 wells1 set3,033
C24776推荐CultureWell Multiwell Chambered Coverslip, 24 mm x 50 mm coverslips, 2 wells1 set3,105
C24779CultureWell Multiwell Chambered Coverslip, 24 mm x 50 mm coverslips, 8 wells1 set2,972
C18139CoverWell Perfusion Chamber Gasket, 8 chambers1 set2,181
C18150推荐CoverWell Incubation Chamber Gasket25 gaskets2,972
C18151CoverWell Incubation Chamber Gasket50 gaskets3,584
C18155CoverWell Incubation Chamber Gasket25 gaskets2,849
C18160CoverWell Imaging Chamber Gasket40 gaskets2,427
C18161CoverWell Imaging Chamber Gasket40 gaskets2,427
C24727CoverWell Imaging Chamber Gasket with Adhesive40 gaskets2,584
P24742Press-to-Seal Silicone Isolator with Adhesive, 24 wells, 2.5 mm x 2.0 mm1 set3,423
P18174Press-to-Seal Silicone Isolator,1 well, 20 mm x 0.5 mm1 set2,618
P24744Press-to-Seal Silicone Isolator with Adhesive, 8 wells, 9 mm x 1.0 mm1 set2,399
P24743Press-to-Seal Silicone Isolator with Adhesive, 8 wells, 9 mm x 0.5 mm1 set2,399
P24740推荐Press-to-Seal Silicone Isolator with Adhesive, 1 well, 20 mm x 0.5 mm1 set1,979
P24745Press-to-Seal Silicone Sheet with Adhesive, 13 cm x 18 cm x 0.5 mm thick1 set4,681
P18178Press-to-Seal Silicone Sheet, 13 cm x 18 cm, 0.5 mm thick1 set3,295
P18179Press-to-Seal Silicone Sheet, 13 cm x 18 cm, 1.0 mm thick1 set4,361
S24737推荐Secure-Seal Spacer, 8 wells, 9 mm diameter, 0.12 mm deep1 set2,972
S24735Secure-Seal Spacer, 1 well, 13 mm diameter, 0.12 mm deep1 set2,399
S24736Secure-Seal Spacer, 1 well, 20 mm diameter, 0.12 mm deep1 set2,484

2固定、透化、封闭

此步骤是为标记细胞做好准备。聚焦目的靶蛋白和细胞结构,同时将其他一切背景排除在图片之外,为获得最佳的成像质量做铺垫。

  • 固定可以锁定细胞状态
  • 透化可使抗体和染色剂进入细胞内
  • 封闭可减少抗体或染料与样品的非特异结合

Invitrogen™ Molecular Probes™ 可提供用于ICC/IF 实验的固定、透化、封闭相关试剂,帮助您获得优化的实验结果

促销代码P2518300

产品名称规格特点货号目录价数量
PBS (Phosphate-Buffered Saline) Tablets100片
  • PBS片剂,可直接溶于去离子水;
  • 每片可配100ml
003002785
Image-iT™ Fixative Solution20 ml
  • 高纯度4%甲醛即用型试剂
  • 无需溶解,中性pH值
  • 优化配方,室温保存
  • 不含甲醇
FB002545
Image-iT™ Fixative Solution5 x 20 mlR378141814
Image-iT™ Fixation/Permeabilization Kit1 kit
  • 可靠:适用大多数细胞,兼容荧光蛋白
  • 便捷:包含固定/透化/封闭/洗涤全套试剂
  • 易用:即用型,无需再优化
R376023792
Image-iT™ FX Signal Enhancer ReadyProbes™ Reagent6 × 2.5 ml
  • 可以抑制由荧光染料带来的非特异性背景
  • 即用型滴瓶包装,室温保存
R371073288
BlockAid™ Blocking Solution50 ml
  • 极低背景:优于传统封闭溶液
  • 即用型:无需稀释或制备储存液
  • 通用全能:可与一抗或二抗等偶联物一起使用,与宿主种属无关
B107102080
Endogenous Biotin Blocking Kit 1 kit
  • 显著提高信噪比,区别细胞靶标
  • 即用型滴瓶包装,快速方便
  • 1小时内明显降低内源性背景
  • 可改善多种传统实验的结果
E213903134

3免疫标记

选择对靶蛋白特异的抗体至关重要,是免疫荧光检测能否成功的一个重要方面。抗体的选择需要考虑:是否经过验证、种属反应性、特异性、克隆、宿主来源、浓度、同行文献引用等。

我们可提供近10万种高品质Invitrogen一抗

  • 经过严格验证的质量保障抗体
  • 覆盖10大主流实验应用、50多个研究领域、超91%人类蛋白组
  • 快捷查找工具:登录thermofisher.com/antibodies

促销代码P2518300

  • Invitrogen 高品质一抗
  • 畅销内参抗体
  • 热门表位标记和融合蛋白抗体

Invitrogen 高品质一抗

货号产品名称规格目录价数量
线粒体
MA1516HIF1A Monoclonal Antibody (mgc3)100 µL5,127
MA3922PLN Monoclonal Antibody (2D12)100 µg5,127
358100COX1 Monoclonal Antibody (COX 111)100 µg4,443
A11133Bovine Serum Albumin Polyclonal Antibody500 µL3,076
PA1037eNOS Polyclonal Antibody100 µL5,127
MA1510Glucocorticoid Receptor Monoclonal Antibody (BuGR2)100 µg5,127
MA512557p53 Monoclonal Antibody (DO-7)500 µL4,443
MA3007HSP70 Monoclonal Antibody (5A5)250 µL5,455
MA1029A488Aconitase 2 Monoclonal Antibody (7G4), Alexa Fluor 48850 µL3,760
MA1930A488ATP Synthase beta Monoclonal Antibody (4.3E8.D10), Alexa Fluor 48850 µL3,760
MA1930A555ATP Synthase beta Monoclonal Antibody (4.3E8.D10), Alexa Fluor 55550 µL3,760
MA1930A647ATP Synthase beta Monoclonal Antibody (4.3E8.D10), Alexa Fluor 64750 µL3,760
MA3012A488HSP60 Monoclonal Antibody (4B9/89), Alexa Fluor 48850 µL3,760
MA338200A488Cytochrome C Monoclonal Antibody (6H2.B4), Alexa Fluor 48850 µL3,760
MA456400A488Mitofilin Monoclonal Antibody (MM#7A-2E4AD5), Alexa Fluor 48850 µL3,760
MA456400A647Mitofilin Monoclonal Antibody (MM#7A-2E4AD5), Alexa Fluor 64750 µL3,760
MA456400A555Mitofilin Monoclonal Antibody (MM#7A-2E4AD5), Alexa Fluor 55550 µL3,760
MA338200A647Cytochrome C Monoclonal Antibody (6H2.B4), Alexa Fluor 64750 µL3,760
MA338200A555Cytochrome C Monoclonal Antibody (6H2.B4), Alexa Fluor 55550 µL3,760
MA3012A647HSP60 Monoclonal Antibody (4B9/89), Alexa Fluor 64750 µL3,760
MA3012A555HSP60 Monoclonal Antibody (4B9/89), Alexa Fluor 55550 µL3,760
MA1029A647Aconitase 2 Monoclonal Antibody (7G4), Alexa Fluor 64750 µL3,760
MA1029A555Aconitase 2 Monoclonal Antibody (7G4), Alexa Fluor 55550 µL3,760
细胞骨架
PA516697Smooth Muscle Actin Polyclonal Antibody500 µL4,443
PA116890gamma Actin Polyclonal Antibody100 µg5,127
MA513539Cytokeratin 17 Monoclonal Antibody (E3)500 µL4,443
PA529003TNNT1 Polyclonal Antibody100 µL5,126
PA522060alpha Tubulin Polyclonal Antibody100 µL5,126
MA1118Xbeta-3 Tubulin Monoclonal Antibody (2G10)20 µL1,901
PA516891alpha Tubulin Polyclonal Antibody500 µL4,443
MA511883A647Vimentin Monoclonal Antibody (V9), Alexa Fluor 64750 µL3,760
MA511883A488Vimentin Monoclonal Antibody (V9), Alexa Fluor 48850 µL3,760
MA138000A488alpha Tubulin Monoclonal Antibody (TU-01), Alexa Fluor 48850 µL4,088
MA322600A488beta Tubulin Monoclonal Antibody (2 28 33), Alexa Fluor 48850 µL3,760
MA322600A647beta Tubulin Monoclonal Antibody (2 28 33), Alexa Fluor 64750 µL3,760
MA322600A555beta Tubulin Monoclonal Antibody (2 28 33), Alexa Fluor 55550 µL3,760
MA138000A647alpha Tubulin Monoclonal Antibody (TU-01), Alexa Fluor 64750 µL3,760
MA138000A555alpha Tubulin Monoclonal Antibody (TU-01), Alexa Fluor 55550 µL3,760
细胞膜
MA1310Estrogen Receptor alpha Monoclonal Antibody (33)100 µg5,127
PA1037eNOS Polyclonal Antibody100 µL5,127
MA170053Complement C3b Monoclonal Antibody (6C9)250 µg5,127
AMC3019TNF alpha Polyclonal Antibody, Biotin100 µg4,443
MA3919SERCA2 ATPase Monoclonal Antibody (2A7-A1)100 µL5,127
PA513483CD154 Polyclonal Antibody400 µL5,126
MA3928A488ATP1A1 Monoclonal Antibody (M7-PB-E9), Alexa Fluor 48850 µL3,760
MA3928A555ATP1A1 Monoclonal Antibody (M7-PB-E9), Alexa Fluor 55550 µL3,760
MA3928A647ATP1A1 Monoclonal Antibody (M7-PB-E9), Alexa Fluor 64750 µL3,760
MA700308A647EGFR Antibody, Alexa Fluor 647 (30H45L8), ABfinity™ Rabbit Monoclonal50 µL3,760
MA700308A555EGFR Antibody, Alexa Fluor 555 (30H45L8), ABfinity™ Rabbit Monoclonal50 µL3,760
MA700308A488EGFR Antibody, Alexa Fluor 488 (30H45L8), ABfinity™ Rabbit Monoclonal50 µL3,760
细胞浆
MA1514AHR Monoclonal Antibody (RPT1)100 µL5,127
MA1744Actin Monoclonal Antibody (mAbGEa)100 µL5,127
MA1516HIF1A Monoclonal Antibody (mgc3)100 µL5,127
MA1310Estrogen Receptor alpha Monoclonal Antibody (33)100 µg5,127
PA1037eNOS Polyclonal Antibody100 µL5,127
MA1510Glucocorticoid Receptor Monoclonal Antibody (BuGR2)100 µg5,127
MA1250CHOP Monoclonal Antibody (9C8)100 µg5,127
PA1027AA488Cyclophilin B Polyclonal Antibody, Alexa Fluor 48850 µL3,760
PA3012A488HSP90 beta Polyclonal Antibody, Alexa Fluor 48850 µL3,760
PA3012A647HSP90 beta Polyclonal Antibody, Alexa Fluor 64750 µL3,760
PA3012A555HSP90 beta Polyclonal Antibody, Alexa Fluor 55550 µL3,760
PA1027AA647Cyclophilin B Polyclonal Antibody, Alexa Fluor 64750 µL3,760
PA1027AA555Cyclophilin B Polyclonal Antibody, Alexa Fluor 55550 µL3,760
MA1065A488Clathrin Heavy Chain Monoclonal Antibody (X22), Alexa Fluor 48850 µL3,760
MA1065A647Clathrin Heavy Chain Monoclonal Antibody (X22), Alexa Fluor 64750 µL3,760
MA1065A555Clathrin Heavy Chain Monoclonal Antibody (X22), Alexa Fluor 55550 µL3,760
溶酶体
MA513324CD68 Monoclonal Antibody (KP1)500 µL4,443
358800CXCR4 Monoclonal Antibody (12G5)100 µg4,443
MA119281CD63 Monoclonal Antibody (MEM-259)100 µg4,443
MA110202CD34 Monoclonal Antibody (QBEnd-10)100 µg2,447
512200LAMP-2A Polyclonal Antibody100 µg4,443
PA1654ALAMP1 Polyclonal Antibody100 µg5,127
MA3067RAB9A Monoclonal Antibody (Mab9)100 µL5,127
MA515825Beclin 1 Monoclonal Antibody (2A4)100 µL5,127
PA514118beta-2 Adrenergic Receptor Polyclonal Antibody400 µL5,126
PA523120Phospho-TLR3 (Tyr759) Polyclonal Antibody20 µg5,127
MA1205A647LAMP2 Monoclonal Antibody (H4B4), Alexa Fluor 64750 µg3,760
MA1205A555LAMP2 Monoclonal Antibody (H4B4), Alexa Fluor 55550 µg3,760
MA1205A488LAMP2 Monoclonal Antibody (H4B4), Alexa Fluor 48850 µg3,760
细胞核
MA31000Lamin A/C Monoclonal Antibody (mab636)200 µL3,760
MA1516HIF1A Monoclonal Antibody (mgc3)100 µL5,127
MA1310Estrogen Receptor alpha Monoclonal Antibody (33)100 µg5,127
MA512789Collagen II Monoclonal Antibody (2B1.5)500 µL5,127
MA1510Glucocorticoid Receptor Monoclonal Antibody (BuGR2)100 µg5,127
MA12022Phospho-Histone H2A.X (Ser140) Monoclonal Antibody (3F2)100 µg5,127
MA702023A488Histone H3 Antibody, Alexa Fluor 488 (17H2L9), ABfinity™ Rabbit Monoclonal50 µL3,760
PA1860A488HDAC1 Polyclonal Antibody, Alexa Fluor 48850 µL3,760
PA1861A488HDAC2 Polyclonal Antibody, Alexa Fluor 48850 µL3,760
PA1861A647HDAC2 Polyclonal Antibody, Alexa Fluor 64750 µL3,760
PA1861A555HDAC2 Polyclonal Antibody, Alexa Fluor 55550 µL3,760
PA1860A647HDAC1 Polyclonal Antibody, Alexa Fluor 64750 µL3,760
PA1860A555HDAC1 Polyclonal Antibody, Alexa Fluor 55550 µL3,760
MA702023A647Histone H3 Antibody, Alexa Fluor 647 (17H2L9), ABfinity™ Rabbit Monoclonal50 µL3,760
MA702023A555Histone H3 Antibody, Alexa Fluor 555 (17H2L9), ABfinity™ Rabbit Monoclonal50 µL3,760
MA332100A488Lamin B2 Monoclonal Antibody (E-3), Alexa Fluor 48850 µL3,760
MA332100A647Lamin B2 Monoclonal Antibody (E-3), Alexa Fluor 64750 µL3,760
MA332100A555Lamin B2 Monoclonal Antibody (E-3), Alexa Fluor 55550 µL3,760
MA325200A488NPM1 Monoclonal Antibody (FC-61991), Alexa Fluor 48850 µL3,760
MA325200A647NPM1 Monoclonal Antibody (FC-61991), Alexa Fluor 64750 µL3,760
MA325200A555NPM1 Monoclonal Antibody (FC-61991), Alexa Fluor 55550 µL3,760
核糖体
701085alpha Synuclein Antibody (14H2L1), ABfinity™ Rabbit Monoclonal100 µg4,443
PA1014AGRP78 Polyclonal Antibody100 µg5,127
131600Ubiquitin Monoclonal Antibody (Ubi-1)100 µg4,443
PA517093RPL26 Polyclonal Antibody100 µL4,443
PA527974RPS3 Polyclonal Antibody100 µL5,126
AHO1232mTOR Monoclonal Antibody (215Q18)100 µg4,443
325800PTEN Monoclonal Antibody (2F4C9)100 µg4,443
MA515140Phospho-S6 (Ser235, Ser236) Monoclonal Antibody (G.22.6)100 µL4,443
PA1013KDEL Polyclonal Antibody100 µg5,127
PA517176RPL13A Polyclonal Antibody100 µL4,443
PA529705GRP78 Polyclonal Antibody100 µL5,126
PA517292RPL7A Polyclonal Antibody100 µL4,443
PA517214RPS3 Polyclonal Antibody100 µL4,443
MA515123S6 Monoclonal Antibody (C.896.4)100 µL4,443
MA515164S6 Monoclonal Antibody (E.573.4)100 µL4,443
PA521390RPS10 Polyclonal Antibody100 µL5,126
PA518529RPL19 Polyclonal Antibody100 µg5,126
MA701374A647S6 Antibody, Alexa Fluor 647 (9H8L2), ABfinity™ Rabbit Monoclonal50 µL3,760
MA701374A555S6 Antibody, Alexa Fluor 555 (9H8L2), ABfinity™ Rabbit Monoclonal50 µL3,760
MA701374A488S6 Antibody, Alexa Fluor 488 (9H8L2), ABfinity™ Rabbit Monoclonal50 µL3,760
中心体
377000PLK1 Monoclonal Antibody (35-206)100 µg4,443
MA1850gamma Tubulin Monoclonal Antibody (4D11)100 µg3,760
PA516762beta Catenin Polyclonal Antibody500 µL5,127
MA12001beta Catenin Monoclonal Antibody (9F2)100 µg4,443
MA1301beta Catenin Monoclonal Antibody (15B8)100 µg4,443
377100PLK1 Monoclonal Antibody (36-298)100 µg4,443
MA1848PLK1 Monoclonal Antibody (13E8)100 µg5,127
PA1126Phospho-PLK1 (Thr210) Polyclonal Antibody200 µg6,138
PA519469beta Catenin Polyclonal Antibody100 µg5,126
MA119421gamma Tubulin Monoclonal Antibody (TU-30)100 µg4,443
MA1300beta Catenin Monoclonal Antibody (6F9)100 µg4,443
PA516429beta Catenin Polyclonal Antibody500 µL5,127
PA534815gamma Tubulin Polyclonal Antibody100 µL5,127
MA1803AKAP450 Monoclonal Antibody (17G10)100 µg5,127
MA514461beta Catenin Monoclonal Antibody (E247)1 mL8,886
MA517152PLK1 Monoclonal Antibody (3C11)100 µg4,443
MA134999beta Catenin Monoclonal Antibody (15B8), Cyanine3100 µL5,127
PA528023PLK1 Polyclonal Antibody100 µL4,443
MA110056beta Catenin Monoclonal Antibody (EM-22)100 µg3,760
PA141319PLK1 Polyclonal Antibody200 µL4,443
中间纤维
MA511883Vimentin Monoclonal Antibody (V9)500 µL4,443
MA511705Filamin A Monoclonal Antibody (FLMN01 (PM6/317))500 µL4,443
MA3745Vimentin Monoclonal Antibody (J144)200 µL4,443
AHO1402Filamin A Monoclonal Antibody (209 No. 13)100 µg4,443
PA513672Cytokeratin 14 Polyclonal Antibody400 µL5,126
MA516421Nestin Monoclonal Antibody (SP103)500 µL6,822
PA516705Desmin Polyclonal Antibody500 µL4,443
PA513663Cytokeratin 10 Polyclonal Antibody400 µL4,443
MA515862Cytokeratin 19 Monoclonal Antibody (1H6)100 µL4,443
MA1110Nestin Monoclonal Antibody (10C2)100 µg5,126
MA513259Desmin Monoclonal Antibody (D33)500 µL4,443
高尔基体
PA1077A647GM130 Polyclonal Antibody, Alexa Fluor 64750 µL3,760
PA1077A555GM130 Polyclonal Antibody, Alexa Fluor 55550 µL3,760
PA1077A488GM130 Polyclonal Antibody, Alexa Fluor 48850 µL3,760
PA1077GM130 Polyclonal Antibody100 µg5,127
A21270Golgin-97 Monoclonal Antibody (CDF4)100 µg3,760
MA122144Golgi protein 58k Monoclonal Antibody (58K-9)100 µL5,455
PA11069Trans-Golgi Network 46 Polyclonal Antibody100 µg5,127
MA3063Trans-Golgi Network 38 Monoclonal Antibody (2F7.1)100 µg5,127
PA3910GRASP65 Polyclonal Antibody100 µL5,127
PA518851MAN2A1 Polyclonal Antibody100 µg5,126
PA549549MAN1A2 Polyclonal Antibody200 µL4,444
PA532780GLG1 Polyclonal Antibody50 µg5,127
PA549655B4GALT1 Polyclonal Antibody400 µL4,444
PA521541GALNT2 Polyclonal Antibody100 µL5,126
PA526385COG2 Polyclonal Antibody400 µL5,127
PA531653GCC1 Polyclonal Antibody100 µL4,443
PA529965gamma Adaptin Polyclonal Antibody100 µL4,443
过氧化物酶体
PA522129PEX19 Polyclonal Antibody100 µL5,126
PA526180SLC25A17 Polyclonal Antibody400 µL5,126
PA530420SLC27A2 Polyclonal Antibody100 µL5,126
PA529183Catalase Polyclonal Antibody100 µL5,126
PA526152ACOT8 Polyclonal Antibody400 µL5,126
PA517271MAVS Polyclonal Antibody100 µL4,443
PA529550MUL1 Polyclonal Antibody100 µL5,126
PA535203ATG7 Polyclonal Antibody400 µL5,127
PA518531Catalase Polyclonal Antibody100 µg4,443
PA1650PMP70 Polyclonal Antibody100 µg5,127
PA517218ATG12 Polyclonal Antibody100 µL3,760
PA1650A488PMP70 Polyclonal Antibody, Alexa Fluor 48850 µL3,760
PA1650A647PMP70 Polyclonal Antibody, Alexa Fluor 64750 µL3,760
PA1650A555PMP70 Polyclonal Antibody, Alexa Fluor 55550 µL3,760
自噬体
PA146286LC3B Polyclonal Antibody100 µL5,127
PA522990LC3A Polyclonal Antibody100 µL5,126
PA1775RAB11B Polyclonal Antibody200 µg5,127
PA116930LC3B Polyclonal Antibody100 µL5,127
700712LC3B Antibody (2H30L32), ABfinity™ Rabbit Monoclonal100 µg4,443
OSA00027WATG12 Polyclonal Antibody150 µL4,443
PA116931LC3A/LC3B Polyclonal Antibody100 µL5,127
PA523180LC3A Polyclonal Antibody100 µg4,443
PA522731LC3A/LC3B Polyclonal Antibody, DyLight 488100 µL5,127
PA520484ATG5 Polyclonal Antibody100 µg5,127
715300RAB11 Polyclonal Antibody100 µg4,443
PA530598LC3B Polyclonal Antibody100 µL5,126
700184RAB11 Antibody (3H18L5), ABfinity™ Rabbit Monoclonal100 µg4,443
PA146178ATG5 Polyclonal Antibody100 µL5,127
PA522939LC3B Polyclonal Antibody, HRP100 µL5,127
PA520688ATG5 Polyclonal Antibody100 µg5,127
PA535201ATG5 Polyclonal Antibody400 µL5,127
PA517218ATG12 Polyclonal Antibody100 µL3,760
外泌体
MA119002CD9 Monoclonal Antibody (MEM-61)100 µg4,443
MA119557CD9 Monoclonal Antibody (MEM-61), FITC100 tests3,760
PA527337HSC70 Polyclonal Antibody100 µL5,126
MA110890HSC70 Monoclonal Antibody (N27F3-4)100 µg5,127
MA126078HSC70 Monoclonal Antibody (1B5)100 µg5,811
MA181488CD81 Monoclonal Antibody (1D6)500 µg6,822
MA110891HSC70 Monoclonal Antibody (BB70)100 µg4,443
MA513548CD81 Monoclonal Antibody (1.3.3.22)500 µL4,443
黑色素体
MA515237Melan-A Monoclonal Antibody (M2-7C10)500 µL5,127
PA518538PMEL Polyclonal Antibody100 µg4,443
MA513232PMEL Monoclonal Antibody (HMB45)500 µL5,127
PA526955DCT Polyclonal Antibody400 µL4,443
MA512293TYRP1 Monoclonal Antibody (TA99)500 µL5,591
MA514168Melan-A Monoclonal Antibody (A103)500 µL4,443
OST00066GTRP1 Polyclonal Antibody500 µg4,443
核内体
MA1066IGF2R Monoclonal Antibody (2G11)100 µg5,127
MA3600Caveolin 1 Monoclonal Antibody (7C8)100 µL4,443
44788GPhospho-EGFR (Tyr1068) Polyclonal Antibody10 blots4,443
MA3924ATP1A1 Monoclonal Antibody (9-A5)100 µL5,127
AMC3019TNF alpha Polyclonal Antibody, Biotin100 µg4,443
PA1064Caveolin 1 Polyclonal Antibody100 µg5,127
MA514794EEA1 Monoclonal Antibody (F.43.1)100 µL4,443
PA3915RAB5 Polyclonal Antibody100 µL5,127
PA3912RAB4A Polyclonal Antibody100 µL4,443
MA1935CFTR Monoclonal Antibody (CF3)100 µL5,127
MA3929ATP1A1 Monoclonal Antibody (M8-P1-A3)100 µg5,127
PA1063AEEA1 Polyclonal Antibody100 µg5,127
AHO1011ErbB2 Monoclonal Antibody (e2-4001)100 µg4,772
PA3850IGF2R Polyclonal Antibody100 µL5,127
MA3928ATP1A1 Monoclonal Antibody (M7-PB-E9)400 µL5,127
MA700184A488RAB11 Antibody, Alexa Fluor 488 (3H18L5), ABfinity™ Rabbit Monoclonal50 µL3,760
胞间连接
MA2607CD26 Monoclonal Antibody (236.3)500 µg5,127
710220Claudin 16 Antibody (10HCLC), ABfinity™ Rabbit Oligoclonal100 µg4,443
131700E-cadherin Monoclonal Antibody (HECD-1)100 µg5,127
PA516867Claudin 3 Polyclonal Antibody500 µL5,127
MA512547E-cadherin Monoclonal Antibody (NCH-38)500 µL5,127
PA534475Pannexin 1 Polyclonal Antibody100 µg5,127
701134E-cadherin Antibody (5H6L18), ABfinity™ Rabbit Monoclonal100 µg4,443
710223Claudin 8 Antibody (6HCLC), ABfinity™ Rabbit Oligoclonal100 µg4,443
700178Claudin 18 Antibody (34H14L15), ABfinity™ Rabbit Monoclonal100 µg4,443
MA511496E-cadherin Monoclonal Antibody (DH01 (DCS-266))500 µL5,127
OSR00136WTRPV4 Polyclonal Antibody100 µL5,126
131900E-cadherin Monoclonal Antibody (ECCD-2)100 µg5,127
MA1159N-cadherin Monoclonal Antibody (13A9)100 µg4,443
MA12002N-cadherin Monoclonal Antibody (8C11)100 µg5,127
333900N-cadherin Monoclonal Antibody (3B9)100 µg4,443
132000ZP-cadherin Monoclonal Antibody (PCD-1)100 µg5,127
蛋白酶体
PA1965PSMC1 Polyclonal Antibody100 µL5,127
PA1978PSMB6 Polyclonal Antibody100 µg5,127
PA1966PSMD4 Polyclonal Antibody200 µL4,443
PA1960PSME1 Polyclonal Antibody100 µg5,127
PA1969PSMC2 Polyclonal Antibody100 µL5,127
PA11960PSMB9 Polyclonal Antibody100 µg5,127
PA1963PSMA1 Polyclonal Antibody100 µg5,127
PA1976PSME2 Polyclonal Antibody100 µL5,127
PA1973PSMD1 Polyclonal Antibody100 µg5,127
PA1961PSME3 Polyclonal Antibody100 µg5,127
PA528030PSMA1 Polyclonal Antibody100 µL5,126
PA1964PSMD2 Polyclonal Antibody100 µL5,127
PA1977PSMB5 Polyclonal Antibody100 µg5,127
PA1974PSMD3 Polyclonal Antibody100 µg5,127
PA1962PSMD2 Polyclonal Antibody100 µL4,443
PA1972PSMB8 Polyclonal Antibody100 µg5,127
PA11962PSMA5 Polyclonal Antibody100 µg5,127
PA530988PSMB7 Polyclonal Antibody100 µL5,126
PA1972A488PSMB8 Polyclonal Antibody, Alexa Fluor 48850 µL3,760
PA1974A488PSMD3 Polyclonal Antibody, Alexa Fluor 48850 µL3,760
PA1978A488PSMB6 Polyclonal Antibody, Alexa Fluor 48850 µL3,760
PA1978A647PSMB6 Polyclonal Antibody, Alexa Fluor 64750 µL3,760
PA1978A555PSMB6 Polyclonal Antibody, Alexa Fluor 55550 µL3,760
PA1974A647PSMD3 Polyclonal Antibody, Alexa Fluor 64750 µL3,760
PA1974A555PSMD3 Polyclonal Antibody, Alexa Fluor 55550 µL3,760
PA1972A647PSMB8 Polyclonal Antibody, Alexa Fluor 64750 µL3,760
PA1972A555PSMB8 Polyclonal Antibody, Alexa Fluor 55550 µL3,760

4多色检测&复染

将特异性结合了一抗的靶点再标记上荧光染料以获得检测信号,根据靶蛋白的表达丰度可选择不同的样本放大信号检测方法以获得最优质图像,常见两类:直接标记和间接标记

  • 高丰度靶蛋白:使用直标一抗,或抗体标记试剂盒标记抗体
  • 中丰度靶蛋白:使用荧光二抗或生物素化二抗
  • 低丰度靶蛋白:使用酶信号放大系统,如SuperBoost

促销代码P2518300

  • 抗体标记试剂盒
  • Alexa Fluor Plus荧光二抗
  • SuperBoost信号放大试剂
  • Streptavidin 偶联物
  • 复染试剂

抗体标记试剂盒

Invitrogen可提供多款抗体标记试剂盒如Zenon™ 系列、APEX™ 系列、SiteClick™ 系列、SAIVI™等, 具体可见thermofisher.com/antibodylabeling

标记IgG 量标记连接推荐抗体标记产品类别
<20 µg位点特异Zenon™ 系列抗体标记试剂盒
<100 µg位点特异SiteClick™ 系列抗体标记试剂盒
10-20 µg传统共价连接APEX™ 系列抗体标记试剂盒
20-100 µg传统共价连接Microscale 系列蛋白标记试剂盒
100 µg传统共价连接Molecular Probes™ 抗体标记试剂盒
1 mg传统共价连接蛋白标记试剂盒
0.5-3 mg传统共价连接SAIVI™ 飞速抗体标记试剂盒

SiteClick™ 系列抗体标记试剂盒选择指南

货号产品名称规格目录价数量
S10467SiteClick™ R-PE Antibody Labeling Kit1 kit5754
S10449SiteClick™ Qdot™ 525 Antibody Labeling Kit1 kit6508
S10450SiteClick™ Qdot™ 565 Antibody Labeling Kit1 kit6495
S10451SiteClick™ Qdot™ 585 Antibody Labeling Kit1 kit6390
S10469SiteClick™ Qdot™ 605 Antibody Labeling Kit1 kit6830
S10452SiteClick™ Qdot™ 625 Antibody Labeling Kit1 kit6609
S10453SiteClick™ Qdot™ 655 Antibody Labeling Kit1 kit6552
S10454SiteClick™ Qdot™ 705 Antibody Labeling Kit1 kit6511
S10455SiteClick™ Qdot™ 800 Antibody Labeling Kit1 kit6510
C10407Click-IT™ Alexa Fluor™ 594 DIBO Alkyne1 kit5590
抗体标记选择荧光染料标记Ex/Em(nm)流式应用成像应用货号目录价数量
APEX™ 系列抗体标记试剂盒Alexa Fluor™ 488496/519A104687844
Alexa Fluor™ 555555/565 A104707708
Alexa Fluor™ 568578/603 A104946356
Alexa Fluor™ 594590/617 A104747781
Alexa Fluor™ 647650/665A104757878
Pacific Blue™ dye416/451A104787806
Biotin-XXNAA104956267
Microscale 系列蛋白标记试剂盒Alexa Fluor™ 488495/519A300068484
Alexa Fluor™ 555555/565 A300078609
Alexa Fluor™ 594590/617 A300088391
Alexa Fluor™ 647650/665A300098481
Biotin-XXNAB300107014
Biotin-XX (biotin quantitation)NAB307568108
Molecular Probes™ 抗体标记试剂盒Alexa Fluor™ 350346/442 A201808572
Alexa Fluor™ 488495/519A201818361
Alexa Fluor™ 532532/553 A201828298
Alexa Fluor™ 546556/573 A201838304
Alexa Fluor™ 555555/565 A201878431
Alexa Fluor™ 568578/603 A201848383
Alexa Fluor™ 594590/617 A201858557
Alexa Fluor™ 647650/665A201868352
Alexa Fluor™ 680684/707A201887001
Alexa Fluor™ 790784/814 A201896004
Pacific Blue™ dye410/455P300137893
Pacific Orange™ dye405/551 P300147932
蛋白标记试剂盒Alexa Fluor™ 350346/442 A101706423
Alexa Fluor™ 488495/519A102358154
Alexa Fluor™ 532532/553 A102367980
Alexa Fluor™ 546556/573 A102377916
Alexa Fluor™ 555555/565 A102748279
Alexa Fluor™ 568578/603 A102388079
Alexa Fluor™ 594590/617 A102398077
Alexa Fluor™ 633621/639A201708108
Alexa Fluor™ 647650/665A201738194
Alexa Fluor™ 660663/690A201717857
Alexa Fluor™ 680679/702 A201727957
Fluorescein494/518F102407184
Oregon Green™ 488496/524O102417155
Pacific Blue™ dye410/455P300127778
Pacific Orange™ dye400/551 P300167805
Texas Red™ dye595/615 T102447272
BiotinNAD206557785

Zenon™ 系列抗体标记试剂盒选择指南

反应性靶标类型产品名称货号目录价数量
MouseIgG1Zenon™ Pacific Blue™ Mouse IgG1 Labeling KitZ250415,593
Zenon™ Alexa Fluor™ 350 Mouse IgG1 Labeling KitZ250006,041
Zenon™ Alexa Fluor™ 405 Mouse IgG1 Labeling KitZ250135,709
Zenon™ Alexa Fluor™ 488 Mouse IgG1 Labeling KitZ250025,898
Zenon™ Alexa Fluor™ 488 Mouse IgG1 Labeling Kit (TSA™)Z250906,229
Zenon™ Alexa Fluor™ 546 Mouse IgG1 Labeling KitZ250045,758
Zenon™ Alexa Fluor™ 555 Mouse IgG1 Labeling KitZ250056,154
Zenon™ Alexa Fluor™ 568 Mouse IgG1 Labeling KitZ250066,103
Zenon™ Alexa Fluor™ 594 Mouse IgG1 Labeling KitZ250076,087
Zenon™ Alexa Fluor™ 647 Mouse IgG1 Labeling KitZ250085,945
Zenon™ Alexa Fluor™ 700 Mouse IgG1 Labeling KitZ250115,654
Zenon™ Alexa Fluor™ 647 R-Phycoerythrin Mouse IgG1 Labeling KitZ250215,575
Zenon™ Alexa Fluor™ 750 Allophycocyanin Mouse IgG1 Labeling KitZ250315,709
Zenon™ Allophycocyanin Mouse IgG1 Labeling KitZ250515,720
Zenon™ R-Phycoerythrin Mouse IgG1 Labeling KitZ250555,750
Zenon™ Tricolor Mouse IgG1 Labeling Kit #1Z250606,012
Zenon™ Horseradish Peroxidase Mouse IgG1 Labeling KitZ250545,991
Zenon™ Biotin-XX Mouse IgG1 Labeling KitZ250525,984
IgG2aZenon™ Alexa Fluor™ 488 Mouse IgG2a Labeling Kit Z251025,818
Zenon™ Alexa Fluor™ 555 Mouse IgG2a Labeling Kit Z251055,673
Zenon™ Alexa Fluor™ 594 Mouse IgG2a Labeling Kit Z251076,092
Zenon™ Alexa Fluor™ 647 Mouse IgG2a Labeling Kit Z251085,909
Zenon™ Allophycocyanin Mouse IgG2a Labeling Kit Z251515,593
Zenon™ Biotin-XX Mouse IgG2a Labeling Kit Z251525,992
Zenon™ Pacific Blue™ Mouse IgG2a Labeling Kit Z251565,684
Zenon™ R-Phycoerythrin Mouse IgG2a Labeling Kit Z251555,565
IgG2bZenon™ Alexa Fluor™ 405 Mouse IgG2b Labeling Kit Z252135,951
Zenon™ Alexa Fluor™ 488 Mouse IgG2b Labeling Kit Z252026,082
Zenon™ Alexa Fluor™ 647 Mouse IgG2b Labeling Kit Z252086,053
Zenon™ R-Phycoerythrin Mouse IgG2b Labeling Kit Z252555,641
HumanIgGZenon™ Alexa Fluor™ 488 Human IgG Labeling Kit Z254026,223
Zenon™ Alexa Fluor™ 594 Human IgG Labeling Kit Z254076,144
Zenon™ Alexa Fluor™ 647 Human IgG Labeling Kit Z254086,271
Zenon™ Allophycocyanin Human IgG Labeling Kit Z254515,675
Zenon™ Biotin-XX Human IgG Labeling Kit Z254525,732
Zenon™ Horseradish Peroxidase Human IgG Labeling Kit Z254546,050
Zenon™ R-Phycoerythrin Human IgG Labeling Kit Z254555,593
RabbitIgGZenon™ Alexa Fluor™ 350 Rabbit IgG Labeling Kit Z253006,053
Zenon™ Alexa Fluor™ 405 Rabbit IgG Labeling Kit Z253135,882
Zenon™ Alexa Fluor™ 488 Rabbit IgG Labeling Kit Z253026,242
Zenon™ Alexa Fluor™ 532 Rabbit IgG Labeling Kit Z253035,976
Zenon™ Alexa Fluor™ 546 Rabbit IgG Labeling Kit Z253046,088
Zenon™ Alexa Fluor™ 555 Rabbit IgG Labeling Kit Z253056,100
Zenon™ Alexa Fluor™ 568 Rabbit IgG Labeling Kit Z253066,080
Zenon™ Alexa Fluor™ 594 Rabbit IgG Labeling Kit Z253075,831
Zenon™ Alexa Fluor™ 647 Rabbit IgG Labeling Kit Z253086,233
Zenon™ Alexa Fluor™ 750 Rabbit IgG Labeling Kit Z253126,094
Zenon™ Allophycocyanin Rabbit IgG Labeling Kit Z253515,486
Zenon™ Biotin-XX Rabbit IgG Labeling Kit Z253526,010
Zenon™ Horseradish Peroxidase Rabbit IgG Labeling Kit Z253546,016
Zenon™ R-Phycoerythrin Rabbit IgG Labeling Kit Z253555,675
Zenon™ Tricolor Rabbit IgG Labeling Kit #1Z253606,184
Zenon™ Tricolor Rabbit IgG Labeling Kit #2Z253706,048
GoatIgGZenon™ Alexa Fluor™ 488 Goat IgG Labeling Kit - 50 LabelingZ256025,982
Zenon™ Alexa Fluor™ 647 Goat IgG Labeling Kit - 50 LabelingZ256085,994

Invitrogen Alexa Fluor荧光二抗

我们可提供单独的Alexa Fluor™染料和Alexa Fluor荧光标记二抗,其荧光信号强度高于其他传统荧光标记物。作为Alexa Fluor染料技术的创始者,我们在荧光二抗方面拥有丰富的经验,提供的产品具有高亮度和光稳定性,完胜传统荧光二抗。在过去二十年的荧光成像领域中,有超过30,000篇出版文章成功应用了Alexa Fluor染料。请登录thermofisher.com/antibodies查询

最新升级版Alexa Fluor Plus标记二抗促销代码P22448
 Alexa Fluor Plus 488Alexa Fluor Plus 555Alexa Fluor Plus 594Alexa Fluor Plus 647Alexa Fluor Plus 680Alexa Fluor Plus 800
Goat anti-RabbitA32731A32732A32740A32733A32734A32735
Goat anti-MouseA32723A32727A32742A32728A32729A32730
Goat anti-ChickenA32931A32932A32759A32933A32934A32935
Goat anti-GoatA32814A32816A32758A32849A32860A32930
Goat anti-RabbitA32790A32794A32754A32795A32802A32808
Goat anti-MouseA32766A32773A32744A32787A32788A32789

超灵敏SuperBoost™ 信号放大产品

Invitrogen™SuperBoost™ 信号放大技术专为低丰度靶标检测设计,将明亮的Alexa Fluor™ 染料与值得信赖的poly-HRP酪胺信号放大技术相结合,提供了一种超高灵敏的检测方法,比常规ICC/IHC/ISH标准方法高10-200倍。

更多信息请查询thermofisher.com/superboost

荧光染料Ex/Em*规格SuperBoost 信号放大货号2019目录价数量
Alexa Fluor 488495/519150 slidesGoat anti - mouse IgGB409126821
50 slidesB409413628
Alexa Fluor 555555/565150 slidesB409136821
Alexa Fluor 594591/617150 slidesB409156821
50 slidesB409423628
Alexa Fluor 647650/668150 slidesB409166821
Biotin-XXNA150 slidesB409115991
Alexa Fluor 488495/519150 slidesGoat anti - rabbit IgGB409226821
50 slidesB409433628
Alexa Fluor 555555/565150 slidesB409236821
Alexa Fluor 594591/617150 slidesB409256821
50 slidesB409443628
Alexa Fluor 647650/668150 slidesB409266821
Biotin-XXNA150 slidesB409215991
Alexa Fluor 488495/519150 slidesStreptavidinB409326821
Alexa Fluor 555555/565150 slidesB409336821
Alexa Fluor 594591/617150 slidesB409356821
Alexa Fluor 647650/668150 slidesB409366821
Biotin-XXNA150 slidesB409316265
Alexa Fluor 350347/442150 slidesTyramide
conjugate
单独装
B409524551
Alexa Fluor 488495/519150 slidesB409534597
Alexa Fluor 546556/573150 slidesB409544551
Alexa Fluor 555555/565150 slidesB409554597
Alexa Fluor 568579/604150 slidesB409564597
Alexa Fluor 594591/617150 slidesB409574597
Alexa Fluor 647650/668150 slidesB409584597
Biotin-XXNA150 slidesB409514597

Streptavidin 偶联物

Invitrogen还可提供多种链霉亲和素的荧光染料偶联物,也可以实现信号放大提高检测灵敏度,广泛应用于荧光成像实验,以检测生物素化的生物分子,例如一抗和二抗、配体和毒素、或原位杂交中的DNA探针等。更多链霉亲和素偶联物选择请查阅:thermofisher.com/streptavidin

Streptavidin 偶联物Ex/Em (nm)规格货号目录价数量
Alexa Fluor 350 Streptavidin346/4421 mgS112493,890
Alexa Fluor 488 Streptavidin495/5191 mgS112233,874
0.5 mLS323544,645
Alexa Fluor 514 Streptavidin518/5401 mgS323533,613
Alexa Fluor 532 Streptavidin530/5541 mgS112243,706
Alexa Fluor 555 Streptavidin555/5651 mgS213813,927
0.5 mLS323554,572
Alexa Fluor 546 Streptavidin556/5731 mgS112253,932
Alexa Fluor 568 Streptavidin578/6031 mgS112263,770
Alexa Fluor 594 Streptavidin590/6171 mgS112273,928
0.5 mLS323564,584
Alexa Fluor 633 Streptavidin632/6471 mgS213753,918
Alexa Fluor 647 Streptavidin650/6681 mgS213743,820
0.5 mLS323574,651
Alexa Fluor 660 Streptavidin663/6901 mgS213773,571
Alexa Fluor 680 Streptavidin679/7021 mgS213783,846
0.5 mLS323584,375
Alexa Fluor 700 Streptavidin702/7231 mgS213833,742
Alexa Fluor 750 Streptavidin749/7751 mgS213843,764

复染

细胞通常还需要用DNA或细胞骨架结合染料进行复染,以识别特定的细胞器或结构,易于识别单个细胞。在设计ICC/IF实验时,关键是要确保复染色剂和所选荧光染料的光谱重叠尽量少,以便能够充分区分彼此,比如,DAPI 应避免与蓝色荧光染料如 Alexa Fluor™ 405, DyLight™ 405等一起使用,更多细胞结构产品信息请查阅:thermofisher.com/cellstructure

复染部位产品名称规格货号目录价数量
细胞核NucBlue™ 固定细胞即用型试剂1 kitR376061429
NucBlue™ 活细胞即用型试剂1 kitR376051426
PI即用型试剂1 kitR371083277
Hoechst 33342100 mgH13991225
Hoechst 33342 -10 mg⁄mL Solution in Water10 mLH35701638
SYTOX™ Green 核酸染料250 µLS70203972
SYTO™ 9 Green Fluorescent 核酸染料100 µLS348544061
SYTO™ 82 Orange Fluorescent 核酸染料250 µLS113634004
TO-PRO™-3 Iodide- 1 mM Solution1 mLT36055199
NucRed™ Dead 647 即用型试剂1 kitR371133161
NucGreen™ Dead 488即用型试剂1 kitR371093191
ReadyProbes™ Cell Viability Imaging Kit, Blue/Green1 kitR376092833
ReadyProbes™ Cell Viability Imaging Kit, Blue/Red1 kitR376102913
DAPI10 mgD13061722
细胞骨架Alexa Fluor™ 350 Phalloidin300 unitsA222818119
Alexa Fluor™ 488 Phalloidin300 unitsA123798040
Alexa Fluor™ 555 Phalloidin300 unitsA340558124
Alexa Fluor™ 594 Phalloidin300 unitsA123818040
Alexa Fluor™ 647 Phalloidin300 unitsA222878040
ActinGreen™ 488 即用型试剂1 kitR371103332
ActinRed™ 555即用型试剂1 kitR371123342
细胞质/膜Wheat Germ Agglutinin, Alexa Fluor™ 350 Conjugate5 mgW112635270
Wheat Germ Agglutinin, Alexa Fluor™ 488 Conjugate5 mgW112615219
Wheat Germ Agglutinin, Alexa Fluor™ 555 Conjugate5 mgW324645265
Wheat Germ Agglutinin, Alexa Fluor™ 594 Conjugate5 mgW112625219
Wheat Germ Agglutinin, Alexa Fluor™ 647 Conjugate5 mgW324665265
线粒体MitoTracker™ Red CMXRos20 x 50 µgM75124404
MitoTracker™ Green FM20 x 50 µgM75144420
MitoTracker™ Red FM20 x 50 µgM224254407
MitoTracker™ Orange CMTMRos20 x 50 µgM75104493
MitoTracker™ Red CMXRos20 x 50 µgM75124404
MitoTracker™ Deep Red FM20 x 50 µgM224264409
MitoTracker™ Orange CM-H2TMRos20 x 50 µgM75115215
MitoTracker™ Red CM-H2XRos20 x 50 µgM75135236
JC-15 mgT31687535

5信号优化

抗淬灭封片剂可以保护样本荧光信号的稳定性,减少光漂白,提升图像质量,获得最佳分辨率,并维持数周乃至数月。更多抗淬灭封片剂信息请查阅thermofisher.com/antifades

选择ProLong™ 或SlowFade™ 抗淬灭封片剂促销代码P2518300

特性硬质或软质折射率样本厚度有机染料效果荧光蛋白效果封片后的载玻片保存是否含核染料规格货号目录价数量
ProLong Glass 硬质,固化固化后约1.52 (与盖玻片、油镜类似)0–150 μm最佳最佳长期 (数周至数月)不含核染料5x2mlP369802,442
10mlP369842,239
2mlP36982693
含核染料5x2mlP369812,544
10mlP369852,341
2mlP36983764
ProLong Diamond 固化后约1.470–10 μm厚度可提供最佳结果;在优化条件下的成像厚度可达30 μm最佳最佳不含核染料5x2mlP369612,296
10mlP369702,085
2mlP36965596
含核染料5x2mlP369622,400
10mlP369712,212
2mlP36966647
ProLong Gold 固化后约1.47良好不推荐不含核染料5x2mlP369342,295
10mlP369302,085
2mlP10144596
含核染料5x2mlP369352,399
10mlP369312,192
2mlP36941647
SlowFade Diamond软质,非固化~1.42最佳最佳短期 (数日至数周)不含核染料5x2mlS369632,208
10mlS369721,995
2mlS36967585
含核染料5x2mlS369642,316
10mlS369732,102
2mlS36968643
SlowFade Gold~1.42最佳不推荐不含核染料5x2mlS369372,207
10mlS369361,976
2mlS36940591
含核染料5x2mlS369392,315
10mlS369382,103
2mlS36942649