Sequencing success from as little as 1 ng FFPE DNA or RNA
Worried about sequencing DNA from FFPE samples or fine-needle aspirates for your research? You shouldn’t be.
With citations in more than 500 peer-reviewed publications, and thousands of labs depending on Ion AmpliSeq™ panels for successful next-generation sequencing library preparation, you can expect success with this exceptional technology.
DNA was prepared from 1 cm2 sections of FFPE liver research samples (n=2) using the Ion AmpliSeq™ Direct FFPE DNA Kit protocol and then quantified using the Invitrogen™ Qubit™ dsDNA HS Assay Kit. Input DNA amounts of 1 ng, 10 ng, or 100 ng were used to generate targeted Ion AmpliSeq™ libraries,** prepared using the Ion AmpliSeq™ Kit for Chef DL8, sequenced using the Ion PGM™ System, and analyzed using Torrent Suite™ Software. Comparable performance was demonstrated for all samples with coverage uniformity† >95%, on-target reads‡ >97%, and strand bias <5%.
Watch how Ion AmpliSeq technology works
This simple yet versatile technology is based on highly multiplexed PCR; watch this video to find out how it works.
Customer success stories
“We’ve created four custom Ion AmpliSeq panels, and all of them are performing extremely well on FFPE-derived DNA.”
— Christopher Corless, MD, PhD, Knight Diagnostic Laboratories, Knight Cancer Institute, Oregon, USA
New Ion AmpliSeq Direct FFPE DNA Kit
Introducing an innovative kit that enables extraction of high-quality DNA from FFPE samples in just 30 minutes, with 10 minutes of hands-on time.
Choose from three different types of Ion AmpliSeq panels
The convenient predesigned panels allow researchers to focus on data generation and analysis, not on the labor-intensive primer design and target selection steps.
Our community panels are designed with input from leading researchers in the scientific community.
If the Ion AmpliSeq™ ready-to-use panels don't match your gene targets then simply select a subset of the targets or enter your specific genomic content online and we will design and create customized Ion AmpliSeq NGS panels just for you.
** An Ion AmpliSeq high-content gene panel, targeting 134 genes and containing 2,530 primer pairs, was used to demonstrate targeted sequencing performance.
† Coverage uniformity = percentage of bases covered at ≥20% of the mean coverage.
‡ On-target reads = percentage of reads that mapped to target regions, out of total mapped reads per run.