从常规 PCR 到高保真 PCR,从热启动 PCR 到多重 PCR,Thermo Scientific 分子生物学产品可满足您不同的 PCR 需求。即日起至2015年8月31日,赛默飞世尔科技推出 PCR 扩增专辑特惠,4 折起,产品覆盖整个 PCR 实验流程。

高质量核酸纯化

Thermo Scientific™ GeneJET™ 核酸纯化方案试剂盒

  • 获得高产量的纯净核酸
  • 方便的离心柱形式,使用值得信赖的硅膜技术
  • 对多种样本类型提供特有的实验
  • 产品组货号产品名称规格2015目录价特惠价
    核酸纯化K0502GeneJET Plasmid Miniprep Kit
    (GeneJET质粒小提试剂盒)
    50 preps¥455¥296
    K0503250 preps¥1,817¥1,181
    K0481GeneJET Plasmid Midiprep Kit
    (GeneJET质粒中提试剂盒)
    25 preps¥2,044¥1,329
    K0482100 preps¥7,268¥4,724
    K0491GeneJET Plasmid Maxiprep Kit
    (GeneJET质粒大提试剂盒)
    10 preps¥1,931¥1,255
    K049225 preps¥4,316¥2,805
    K0731GeneJET RNA Purification Kit
    (GeneJET RNA纯化试剂盒)
    50 preps¥2,158¥1,403
    K0732250 preps¥8,630¥5,609
    K0801GeneJET Plant RNA Purification Kit
    (GeneJET 植物RNA纯化试剂盒)
    50 preps¥2,271¥1,476
    K0802250 preps¥9,085¥5,905
    K0721GeneJET Genomic DNA Purification Kit
    (GeneJET 基因组DNA纯化试剂盒)
    50 preps¥1,158¥753
    K0722250 preps¥5,224¥3,395
    K0791GeneJET Plant Genomic DNA Purification Kit
    (GeneJET 植物基因组DNA纯化试剂盒)
    50 preps¥1,420¥923
    K0792250 preps¥6,245¥4,060
    K0701GeneJET PCR Purification Kit
    (GeneJET PCR产物纯化试剂盒)
    50 preps¥455¥296
    K0702250 preps¥1,817¥1,181
    K0691GeneJET Gel Extraction Kit
    (GeneJET 凝胶回收试剂盒)
    50 preps¥455¥296
    K0692250 preps¥1,817¥1,181
    K0831GeneJET Gel Extraction and DNA Cleanup Micro Kit
    (GeneJET 凝胶回收及DNA纯化微型试剂盒)
    50 preps¥658¥428
    K0832250 preps¥3,304¥2,148

    高保真 PCR 的金标准

    Thermo Scientific™ Phusion™ DNA 聚合酶

  • 确保极为准确及快速的扩增
  • 极少的酶量也能进行强劲扩增,获得高产量
  • 热启动形式进一步确保高特异性
  • 产品组货号产品名称规格2015目录价特惠价
    高保真PCRF-530SPhusion High-Fidelity DNA Polymerase
    (Phusion高保真DNA聚合酶)
    100 U¥979¥588
    F-530L500 U ¥3,918¥2,351
    F-534SPhusion Green High-Fidelity DNA Polymerase
    (Phusion高保真DNA聚合酶,绿色缓冲液)
    100 U¥979¥588
    F-534L500 U¥3,918¥2,351
    F-532SPhusion High-Fidelity PCR Master Mix with GC Buffer
    (Phusion高保真PCR预混液,含GC缓冲液)
    100 x 50 μL rxns¥1,588¥953
    F-532L500 x 50 μL rxns¥6,353¥3,812
    F-531SPhusion High-Fidelity PCR Master Mix with HF Buffer
    (Phusion高保真PCR预混液,含HF缓冲液)
    100 x 50 μL rxns¥1,588¥953
    F-531L500 x 50 μL rxns¥6,353¥3,812
    F-549SPhusion Hot Start II High-Fidelity DNA Polymerase
    (Phusion Hot Start II高保真DNA聚合酶)
    100 U ¥1,189¥714
    F-549L500 U ¥4,755¥2,853
    F-537SPhusion Green Hot Start II High-Fidelity DNA Polymerase
    (Phusion Hot Start II高保真DNA聚合酶,绿色缓冲液)
    100 U¥1,189¥714
    F-537L500 U¥4,755¥2,853
    F-565SPhusion Hot Start II High-Fidelity PCR Master Mix
    (Phusion Hot Start II高保真PCR预混液)
    100 x 50 µL rxns¥1,641¥984
    F-565L500 x 50 µL rxns¥6,562¥3,937
    F-566SPhusion Green Hot Start II High-Fidelity PCR Master Mix
    (Phusion Hot Start II高保真PCR预混液,绿色缓冲液)
    100 x 50 µL rxns¥1,641¥984
    F-566L500 x 50 µL rxns¥6,562¥3,937
    F-548SPhusion Flash High-Fidelity PCR Master Mix
    (Phusion 快速高保真PCR预混液)
    100 x 20 µL rxns¥742¥445
    F-548L500 x 20 µL rxns¥2,967¥1,780

    含尿嘧啶模板的 PCR 扩增

    Thermo Scientific™ Phusion U™ DNA 聚合酶

  • 实现对含尿嘧啶模板的准确扩增
  • 在 DNA 扩增延伸中可有效嵌入 dUTP
  • 强劲性能:高产量及高特异性
  • 产品组货号产品名称规格2015目录价特惠价
    Phusion UF-555SPhusion U Hot Start DNA Polymerase
    (Phusion U热启动DNA聚合酶)
    100 U¥1,334¥801
    F-555L500 U¥5,302¥3,181
    F-556SPhusion U Green Hot Start DNA Polymerase
    (Phusion U热启动DNA聚合酶,绿色缓冲液)
    100 U¥1,334¥801
    F-556L500 U¥5,302¥3,181
    F-533SPhusion U Hot Start PCR Master Mix
    (Phusion U热启动PCR预混液)
    100 x 50 µL rxns¥1,906¥1,144
    F-533L500 x 50 µL rxns¥7,581¥4,549

    轻松进行多重 PCR

    Thermo Scientific™ Phusion U™ 多重PCR预混液

  • 可同时扩增多达 20 个目的基因,每个基因片段长达 2.5 kb
  • 即使扩增 GC 含量丰富或不纯的模板,也能获得满意的结果
  • 对于所需的反应条件只需极少的优化
  • 产品组货号产品名称规格2015目录价特惠价
    多重PCRF-562SPhusion U Multiplex PCR Master Mix
    (Phusion U多重PCR预混液)
    100 x 50 µL rxns¥1,880¥1,128
    F-562L500 x 50 µL rxns¥7,476¥4,486
    F-564SPhusion U Green Multiplex PCR Master Mix
    (Phusion U多重PCR预混液,绿色缓冲液)
    100 x 50 µL rxns¥1,880¥1,128
    F-564L500 x 50 µL rxns¥7,476¥4,486

    快速强劲的热启动 DNA 聚合酶

    Thermo Scientific™ Phire™ Hot Start II 热启动 DNA 聚合酶

  • 采用融合蛋白技术,适用于常规 PCR 扩增
  • 经研发适用于快速、高产量和扩增长片段的 PCR
  • 具有高抑制剂耐受能力,只需极少的反应条件优化
  • 产品组货号产品名称规格2015目录价特惠价
    热启动PCRF-122SPhire Hot Start II DNA Polymerase
    (Phire Hot Start II DNA聚合酶)
    200 x 50 µL rxns¥1,046¥628
    F-122L1000 x 50 µL rxns¥4,184¥2,511
    F-124SPhire Green Hot Start II DNA Polymerase
    (Phire Hot Start II DNA聚合酶,绿色缓冲液)
    200 x 50 µL rxns¥1,041¥625
    F-124L1000 x 50 µL rxns¥4,149¥2,489
    F-125SPhire Hot Start II PCR Master Mix
    (Phire Hot Start II PCR预混液)
    200 x 50 µL rxns¥1,675¥1,005
    F-125L1000 x 50 µL rxns¥6,665¥3,999
    F-126SPhire Green Hot Start II PCR Master Mix
    (Phire Hot Start II PCR预混液,绿色缓冲液)
    200 x 50 µL rxns¥1,675¥1,005
    F-126L1000 x 50 µL rxns¥6,665¥3,999

    直接 PCR—扩增无需核酸纯化

    Thermo Scientific™ 直接 PCR 预混液

  • 基于耐抑制剂的融合 DNA 聚合酶
  • 从未纯化样本中直接进行 PCR —无需提取和纯化 DNA
  • PCR 扩增时间极短
  • 使用绿色缓冲液,PCR 产物可以直接上样进行凝胶电泳
  • 产品组货号产品名称规格2015目录价特惠价
    直接PCRF-170SPhire Tissue Direct PCR Master Mix
    (Phire组织直接PCR预混液)
    250 x 20 µL rxns¥1,351¥811
    F-170L1250 x 20 µL rxns¥5,759¥3,455
    F-160SPhire Plant Direct PCR Master Mix
    (Phire植物直接PCR预混液)
    250 x 20 µL rxns¥1,104¥662
    F-160L1250 x 20 µL rxns¥4,814¥2,888
    F-175SPhusion Blood Direct PCR Master Mix
    (Phire血液直接PCR预混液)
    100 x 20 µL rxns¥874¥525
    F-175L500 x 20 µL rxns¥3,498¥2,099

    高效反转录试剂盒

    Thermo Scientific™ Maxima™ 反转录酶

  • 可合成长达 20 kb 的全长 cDNA
  • 良好的热稳定性—在 42°C- 65°C 间高效合成 DNA
  • 高效快速—15-30 分钟即可完成 cDNA 合成,5 分钟可合成 7.5 kb 的 cDNA
  • 高灵敏度—可用不同量的总 RNA(1 pg - 5ug)为模板进行重复性好的 cDNA 合成
  • 增强的抗反应抑制剂能力
  • 产品组货号产品名称规格2015目录价
    Maxima反转录K1641Maxima First Strand cDNA Synthesis Kit for RT-qPCR50 reactions¥2,981
    K1642Maxima First Strand cDNA Synthesis Kit for RT-qPCR200 reactions¥8,942
    K1651Maxima H Minus First Strand cDNA Synthesis Kit20 reactions¥1,157
    K1652Maxima H Minus First Strand cDNA Synthesis Kit100 reactions¥4,626

    DNA Ladders—快速准确的条带分析

    Thermo Scientific™ GeneRuler™ DNA Ladders

  • 宽范围,清晰明亮的参考条带
  • 方便用于 DNA 定量
  • 提供 6 X loading dye
  • Thermo Scientific™ FastRuler™ DNA Ladders

  • 适用于短距离电泳,快速电泳分离
  • 即用型 — 与上样染液预混
  • 产品组货号产品名称规格2015目录价特惠价
    DNA LaddersSM1123FastRuler High Range DNA Ladder, ready-to-use2 x 500 µL¥656¥426
    SM1103FastRuler Low Range DNA Ladder, ready-to-use2 x 500 µL¥656¥426
    SM1113FastRuler Middle Range DNA Ladder, ready-to-use1000 µL¥656¥426
    SM1233FastRuler Ultra Low Range DNA Ladder, ready-to-use2 x 500 µL¥775¥504
    SM0241GeneRuler 100 bp DNA Ladder50 µg¥238¥155
    SM0242GeneRuler 100 bp DNA Ladder5 x 50 µg¥954¥620
    SM0243GeneRuler 100 bp DNA Ladder, RTU50 µg¥262¥171
    SM0321GeneRuler 100 bp Plus DNA Ladder50 µg¥298¥193
    SM0322GeneRuler 100 bp Plus DNA Ladder5 x 50 µg¥1,193¥775
    SM0323GeneRuler 100 bp Plus DNA Ladder, RTU50 µg¥322¥209
    SM0311GeneRuler 1 kb DNA Ladder5 x 50 µg¥596¥388
    SM0312GeneRuler 1 kb DNA Ladder25 x 50 µg¥2,385¥1,550
    SM0313GeneRuler 1 kb DNA Ladder, RTU5 x 50 µg¥632¥411
    SM1331GeneRuler 1 kb Plus DNA Ladder5 x 50 µg¥1,312¥853
    SM1332GeneRuler 1 kb Plus DNA Ladder25 x 50 µg¥5,247¥3,410
    SM1333GeneRuler 1 kb Plus DNA Ladder, RTU5 x 50 µg¥1,788¥1,162
    R06116X DNA Loading Dye5 x 1.0 mL¥298¥193
    R06316X Orange DNA Loading Dye5 x 1.0 mL¥298¥193

    **RTU = ready-to-use,即用

    质优 PCR 耗材

    Thermo Scientific™ 塑料耗材

  • 与主流 PCR 仪器兼容的反应板、反应管和封口产品
  • 洁净空间生产 — 经鉴定不含 DNA, RNases 和 DNases
  • 统一的孔壁实现最大化且一致的热传递
  • 产品组货号产品名称规格2015目录价特惠价
    PCR&qPCR耗材 PCR、qPCR八联排管&盖   
    AB-1771 ABgene Strips of 8 Low Profile Tubes (White) & Ultra Clear Cap Strips
    八联排管&白色&超透明盖,低容 0.2ml
    250 strips¥1,619¥972
    AB-1770ABgene Strips of 8 Low Profile Tubes & Ultra Clear Cap Strips
    八联排管&超透明盖,低容 0.2ml
    250 strips¥1,489¥1,042
    AB-1182ABgene Strips of 8 Tubes & Flat Caps
    八联排管&平盖 0.2ml
    250 strips¥1,807¥1,084
    AB-0266ABgene Strips of 8 Tubes & Domed Caps
    八联排管&圆盖 0.2ml
    250 strips¥1,953¥977
    AB-0264ABgene Strips of 8 Tubes
    八联排管&不含管盖 0.2ml
    250 strips¥1,738¥782
    AB-0866ABgene Ultra Clear Cap Strips
    超透明联排管盖
    120 strips¥351¥193
    96孔板、粘性封口膜 
    AB-080096-well ABgene Low Profile PCR Plate, Skirted
    全裙边96孔板,低容,最大体积 0.2ml
    25 plates¥936¥515
    AB-060096-well ABgene PCR Plate, Non-Skirted
    无裙边96孔板,标准,最大体积 0.3ml
    25 plates¥871¥479
    AB-0558Adhesive PCR Seals
    粘附型PCR板封口膜,透明
    100 sheets¥1,169¥585
    AB-1170ABsolute qPCR Seals
    粘附型qPCR板封口膜,透明
    50 sheets¥1,348¥539
    AB-0626Adhesive PCR Foils
    粘附型PCR板密封箔片,可穿刺
    100 sheets¥908¥499
    96深孔储存板&封口垫 
    AB-079696-Well Standard Microplate (1 bag of 100 plates)
    96孔圆孔U型底储存板 0.33ml
    100 plates¥1,636¥1,063
    AB-0765ABgene 0.8 ml Storage Plate (Individually wrapped)
    96孔圆孔锥型底储存板 0.8ml
    50 plates¥2,362¥1,535
    AB-1127ABgene 1.2 ml Square Well Low Profile Storage Plate (Individually wrapped)
    96孔方孔U型底储存板 1.2ml
    50 plates¥1,538¥1,000
    AB-0564ABgene 1.2 ml Storage Plate (Individually wrapped)
    96孔圆孔U型底储存板 1.2ml
    50 plates¥2,514¥1,634
    AB-0932ABgene 2.2 ml Storage Plate MKII (Individually wrapped)
    96孔方孔锥型底储存板 2.2ml
    50 plates¥2,129¥1,490
    AB-0661ABgene 2.2 ml Storage Plate (Individually wrapped)
    96孔方孔U型底储存板 2.2ml
    50 plates¥2,344¥1,406
    AB-0674Sealing Mats (Round Cap, Autoclavable)
    圆孔型密封垫,耐高压蒸汽灭菌
    50 mats¥1,515¥909
    AB-0566Sealing Mat (Round Cap, Pierceable)
    圆孔型密封垫,可穿刺
    50 mats¥1,139¥798
    384孔板 
    AB-1384PCR Plate, 384-well, standard
    384孔板,全裙边,孔最大体积40µl,工作体积25µl
    50 plates¥1,766¥1,148

    高硬度 PCR 反应板

    Thermo Scientific™ Armadillo™ PCR 反应板

  • 用于自动化实验的 96 孔和 384 孔 PCR 反应板
  • 聚碳酸酯板框可耐受因热循环造成的板卷曲
  • 增强了机械操作处理的稳定性
  • 多种板框颜色选择,都可提供透明孔板(用于 PCR)和白色孔板(用于 qPCR)
  • 产品组货号产品名称规格2015目录价特惠价
    PCR&qPCR耗材 Armadillo PCR&qPCR耗材   
    AB-2396Armadillo 96-Well PCR Plate (Clear, Clear Wells) 25 plates ¥967¥580
    BC-2396Armadillo 96-Well PCR Plate, Barcoded (Clear, Clear Wells)25 plates¥1,081¥649
    AB-2596Armadillo 96-Well Plate, Clear Semi-Skirted, Clear Wells25 plates¥994¥596
    BC-2596Armadillo 96-Well Plate, Clear Semi-Skirted, Clear Wells, Barcoded25 plates¥1,094¥656
    AB-2496Armadillo 96-Well Plate, Clear Semi-Skirted, Low Profile, Clear Wells25 plates¥979¥587
    BC-2496Armadillo 96-Well Plate, Clear Semi-Skirted, Low Profile, Clear Wells, Barcoded25 plates¥1,071¥642
    AB-2384Armadillo 384-Well PCR Plate (Clear, Clear Wells)50 plates¥2,540¥1,524
    BC-2384Armadillo 384-Well PCR Plate, Barcoded (Clear, Clear Wells)50 plates¥2,789¥1,674
    AB-3396Armadillo 96-Well PCR Plate (Clear, White Wells)25 plates¥1,081¥649
    BC-3396Armadillo 96-Well PCR Plate, Barcoded (Clear, White Wells)25 plates¥1,183¥710
    AB-3596Armadillo 96-Well Plate, Clear Semi-Skirted, White Wells25 plates¥1,094¥656
    BC-3596Armadillo 96-Well Plate, Clear Semi-Skirted, White Wells, Barcoded25 plates¥1,201¥720
    AB-3496Armadillo 96-Well Plate, Clear Semi-Skirted, Low Profile, White Wells25 plates¥1,071¥642
    BC-3496Armadillo 96-Well Plate, Clear Semi-Skirted, Low Profile, White Wells, Barcoded25 plates¥1,178¥707
    AB-3384Armadillo 384-Well PCR Plate (Clear, White Wells)50 plates¥2,789¥1,674
    BC-3384Armadillo 384-Well PCR Plate, Barcoded (Clear, White Wells)50 plates¥3,066¥1,840

    选择彩色反应板时请在货号后添加相应后缀:/B 绿色,/G 橙色,/R 红色,/Y 黄色,如 AB-2496/B