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应用领域

[经典文献] Mass-spectrometric exploration of proteome structure and function. Nature, 2016, 537, 347. (Ruedi Aebersold 和 Matthias Mann 的蛋白质组学综述)

[经典文献] Protein Analysis by Shotgun/Bottom-up Proteomics. Chemical Reviews, 2013, 113, 2343. (John Yates 实验室对shotgun蛋白质组学的详细介绍)

样品前处理

[经典文献] Universal sample preparation method for proteome analysis. Nature Methods, 2009, 6, 359. (基于超滤管的蛋白质组学样品制备方法)

[经典文献] In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nature Protocols, 2007, 1, 2856. (胶内酶解方法)

[经典文献] Plasma Proteome Profiling to Assess Human Health and Disease. Cell Systems, 2016, 2, 185. (微量血浆样品制备方法)

[应用报告] 不同蛋白提取液对动物组织全蛋白质组鉴定的影响

[产品手册] 蛋白质组学样品制备和定量产品手册

仪器采集

[应用报告] A robust and sensitive capillary flow LC - high-resolution accurate-mass MS platform for discovery and targeted proteomics.

[经典文献] Electrostatic Axially Harmonic Orbital Trapping: A High-Performance Technique of Mass Analysis. Anal. Chem., 2000, 72, 1156. (Orbitrap质量分析器的原理)

[经典文献] Mass spectrometry-based proteomics using Q Exactive, a high-performance benchtop quadrupole Orbitrap mass spectrometer. Molecular & Cellular Proteomics, 2011, 10, M111.011015. (Q Exactive系列仪器原理)

[经典文献] Novel Parallelized Quadrupole/Linear Ion Trap/Orbitrap Tribrid Mass Spectrometer Improving Proteome Coverage and Peptide Identification Rates. Anal. Chem., 2013, 85, 11710. (Orbitrap Fusion系列仪器原理)

[应用报告] Increased MS Protein Identification Rates Using 75 cm Long nano LC C18 Separation Columns: Pushing the Limits of Bottom-Up Proteomics.

数据分析和生物信息

[仪器软件使用] Proteome Discoverer 2.1 中文版使用手册

[应用报告] An Information System Enabling the Organization and Automatic Annotation of Proteomic Experiments with User-specific Proteins.

蛋白质组学经典文献

[经典文献] The One Hour Yeast Proteome. Mol Cell Proteomics, 2014, 13, 339. (一小时酵母蛋白组)

[经典文献] A draft map of the human proteome. Nature, 2014, 509, 575. (人类蛋白质组草图)

[经典文献] Mass-spectrometry-based draft of the human proteome. Nature, 2014, 509, 582. (人类蛋白质组草图)

[评述] Hela、Yeast 与 Orbitrap Fusion 的一小时

TMT

[产品手册] 定量蛋白质组学产品手册

[产品手册] TMT 产品手册

[经典文献] A draft map of the mouse pluripotent stem cell spatial proteome. Nat. Commun., 2016, 7, 8992. (TMT用于蛋白在不同细胞器的定位研究)

[经典文献] Quantitative temporal viromics: an approach to investigate host-pathogen interaction. Cell, 2014, 157, 1460. (TMT研究宿主-病毒相互作用)

[经典文献] Tandem Mass Tags: A Novel Quantification Strategy for Comparative Analysis of Complex Protein Mixtures by MS/MS. Anal. Chem., 2003, 75, 1895. (TMT原始论文)

[经典文献] Increasing the Multiplexing Capacity of TMTs Using Reporter Ion Isotopologues with Isobaric Masses. Anal. Chem., 2012, 84, 7469. (TMT10原始论文)

[经典文献] MultiNotch MS3 Enables Accurate, Sensitive, and Multiplexed Detection of Differential Expression across Cancer Cell Line Proteomes. Anal. Chem., 2014, 86, 7150. (SPS-MS3原始论文)

[评述] “尾巴”尖上的蛋白组学 (TMT 研究蛋白 N-末端组)

[评述] hyperLOPIT给你一双用于细胞器蛋白定位的“火眼金睛” (TMT 用于蛋白的亚细胞定位定量研究)

[评述] 让“鱼与熊掌兼得”不再是传说--基于Orbitrap Fusion的TMT SPS MS3定量技术 (TMT 高级定量方法简介)

[视频讲座] Dr. Noah Dephoure - Multiplexed Quantitative Analysis of PTMs for Monitoring Changes in Protein Interactions and Subcellular Localization

[视频讲座] Dr. Robert Everley - Multiplexing Targeted Proteomics Assays: Triggered by Offset Multiplexed Accurate mass High resolution Absolute Quantification (TOMAHAQ) on a Lumos Tribrid

[视频讲座] Dr. Wilhelm Haas - High-Throughput Multiplexed Quantitative Proteomics and Phosphoproteomics to Understand Cancer Drug Resistance

[视频讲座] Robust Classification of Stem Cell Protein Localization by SPS-MS3

[视频讲座] 定量蛋白质组学的新高度——从shotgun、到DIA、再到target quan完整解决方案

[仪器软件方法] Q Exactive 系列仪器中 TMT 定量工作流程的仪器参数优化和 Proteome Discoverer 2.1 软件数据处理方法

[应用报告] Evaluation of search engines for phosphopeptide identification and quantitation

[应用报告] Improved Sample Preparation Workflows for Protein Discovery and Quantification in Pathway Analysis (进行定量磷酸化蛋白质组的样品前处理和分级策略比较)

[应用报告] Orbitrap Fusion TMT MS2和MS3定量比较

[应用报告] Sensitive and Accurate Quantitation of Phosphopeptides Using TMT Isobaric Labeling Technique (TMT 进行磷酸化定量蛋白质组研究的 SPS MS3 定量策略比较)

DIA

[解决方案] 数据非依赖采集(DIA)解决方案

[评述] 赛默飞推出数据非依赖采集(DIA)解决方案

[应用报告] Q Exactive HF DIA实现高通量酵母蛋白质组定量分析

[应用报告] Q Exactive HF DIA实现高通量人蛋白质组定量分析

[应用报告] Q Exactive HF DIA实现高通量血清蛋白质组定量分析

[应用报告] Large-Scale Proteome and Phosphoproteome Quantification by Data Independent Acquisition on Ultra-High Field Orbitrap Mass Spectrometers

[应用报告] MS1-Based Quantification Optimization on DIA Methods on a Quadrupole-Orbitrap Mass Spectrometer

[应用报告] Q Exactive HF 一小时 DDA 鉴定 DIA 定量 4000 个 Hela 蛋白

[应用报告] 精确修饰位点谱图库的建立与磷酸化蛋白质组的 DIA 解析

PRM

[应用报告] 应用Target MS2 方法对大鼠血浆中人重组干扰素进行绝对定量的测试方案

[应用报告] Monitoring of the AKT/mTOR pathway using Internal Standard Triggered-Parallel Reaction Monitoring (IS-PRM)

蛋白质组学概述

[经典文献] Mass-spectrometric exploration of proteome structure and function. Nature, 2016, 537, 347. (Ruedi Aebersold 和 Matthias Mann 的蛋白质组学综述)

[经典文献] Protein Analysis by Shotgun/Bottom-up Proteomics. Chemical Reviews, 2013, 113, 2343. (John Yates 实验室对shotgun蛋白质组学的详细介绍)

样品前处理

[经典文献] Universal sample preparation method for proteome analysis. Nature Methods, 2009, 6, 359. (基于超滤管的蛋白质组学样品制备方法)

[经典文献] In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nature Protocols, 2007, 1, 2856. (胶内酶解方法)

[经典文献] Plasma Proteome Profiling to Assess Human Health and Disease. Cell Systems, 2016, 2, 185. (微量血浆样品制备方法)

[应用报告] 不同蛋白提取液对动物组织全蛋白质组鉴定的影响

[产品手册] 蛋白质组学样品制备和定量产品手册

仪器采集

[应用报告] A robust and sensitive capillary flow LC - high-resolution accurate-mass MS platform for discovery and targeted proteomics.

[经典文献] Electrostatic Axially Harmonic Orbital Trapping: A High-Performance Technique of Mass Analysis. Anal. Chem., 2000, 72, 1156. (Orbitrap质量分析器的原理)

[经典文献] Mass spectrometry-based proteomics using Q Exactive, a high-performance benchtop quadrupole Orbitrap mass spectrometer. Molecular & Cellular Proteomics, 2011, 10, M111.011015. (Q Exactive系列仪器原理)

[经典文献] Novel Parallelized Quadrupole/Linear Ion Trap/Orbitrap Tribrid Mass Spectrometer Improving Proteome Coverage and Peptide Identification Rates. Anal. Chem., 2013, 85, 11710. (Orbitrap Fusion系列仪器原理)

[应用报告] Increased MS Protein Identification Rates Using 75 cm Long nano LC C18 Separation Columns: Pushing the Limits of Bottom-Up Proteomics.

数据分析和生物信息

[仪器软件使用] Proteome Discoverer 2.1 中文版使用手册

[应用报告] An Information System Enabling the Organization and Automatic Annotation of Proteomic Experiments with User-specific Proteins.

蛋白质组学经典文献

[经典文献] The One Hour Yeast Proteome. Mol Cell Proteomics, 2014, 13, 339. (一小时酵母蛋白组)

[经典文献] A draft map of the human proteome. Nature, 2014, 509, 575. (人类蛋白质组草图)

[经典文献] Mass-spectrometry-based draft of the human proteome. Nature, 2014, 509, 582. (人类蛋白质组草图)

[评述] Hela、Yeast 与 Orbitrap Fusion 的一小时

定量蛋白质组学

TMT

[产品手册] 定量蛋白质组学产品手册

[产品手册] TMT 产品手册

[经典文献] A draft map of the mouse pluripotent stem cell spatial proteome. Nat. Commun., 2016, 7, 8992. (TMT用于蛋白在不同细胞器的定位研究)

[经典文献] Quantitative temporal viromics: an approach to investigate host-pathogen interaction. Cell, 2014, 157, 1460. (TMT研究宿主-病毒相互作用)

[经典文献] Tandem Mass Tags: A Novel Quantification Strategy for Comparative Analysis of Complex Protein Mixtures by MS/MS. Anal. Chem., 2003, 75, 1895. (TMT原始论文)

[经典文献] Increasing the Multiplexing Capacity of TMTs Using Reporter Ion Isotopologues with Isobaric Masses. Anal. Chem., 2012, 84, 7469. (TMT10原始论文)

[经典文献] MultiNotch MS3 Enables Accurate, Sensitive, and Multiplexed Detection of Differential Expression across Cancer Cell Line Proteomes. Anal. Chem., 2014, 86, 7150. (SPS-MS3原始论文)

[评述] “尾巴”尖上的蛋白组学 (TMT 研究蛋白 N-末端组)

[评述] hyperLOPIT给你一双用于细胞器蛋白定位的“火眼金睛” (TMT 用于蛋白的亚细胞定位定量研究)

[评述] 让“鱼与熊掌兼得”不再是传说--基于Orbitrap Fusion的TMT SPS MS3定量技术 (TMT 高级定量方法简介)

[视频讲座] Dr. Noah Dephoure - Multiplexed Quantitative Analysis of PTMs for Monitoring Changes in Protein Interactions and Subcellular Localization

[视频讲座] Dr. Robert Everley - Multiplexing Targeted Proteomics Assays: Triggered by Offset Multiplexed Accurate mass High resolution Absolute Quantification (TOMAHAQ) on a Lumos Tribrid

[视频讲座] Dr. Wilhelm Haas - High-Throughput Multiplexed Quantitative Proteomics and Phosphoproteomics to Understand Cancer Drug Resistance

[视频讲座] Robust Classification of Stem Cell Protein Localization by SPS-MS3

[视频讲座] 定量蛋白质组学的新高度——从shotgun、到DIA、再到target quan完整解决方案

[仪器软件方法] Q Exactive 系列仪器中 TMT 定量工作流程的仪器参数优化和 Proteome Discoverer 2.1 软件数据处理方法

[应用报告] Evaluation of search engines for phosphopeptide identification and quantitation

[应用报告] Improved Sample Preparation Workflows for Protein Discovery and Quantification in Pathway Analysis (进行定量磷酸化蛋白质组的样品前处理和分级策略比较)

[应用报告] Orbitrap Fusion TMT MS2和MS3定量比较

[应用报告] Sensitive and Accurate Quantitation of Phosphopeptides Using TMT Isobaric Labeling Technique (TMT 进行磷酸化定量蛋白质组研究的 SPS MS3 定量策略比较)

DIA

[解决方案] 数据非依赖采集(DIA)解决方案

[评述] 赛默飞推出数据非依赖采集(DIA)解决方案

[应用报告] Q Exactive HF DIA实现高通量酵母蛋白质组定量分析

[应用报告] Q Exactive HF DIA实现高通量人蛋白质组定量分析

[应用报告] Q Exactive HF DIA实现高通量血清蛋白质组定量分析

[应用报告] Large-Scale Proteome and Phosphoproteome Quantification by Data Independent Acquisition on Ultra-High Field Orbitrap Mass Spectrometers

[应用报告] MS1-Based Quantification Optimization on DIA Methods on a Quadrupole-Orbitrap Mass Spectrometer

[应用报告] Q Exactive HF 一小时 DDA 鉴定 DIA 定量 4000 个 Hela 蛋白

[应用报告] 精确修饰位点谱图库的建立与磷酸化蛋白质组的 DIA 解析

PRM

[应用报告] 应用Target MS2 方法对大鼠血浆中人重组干扰素进行绝对定量的测试方案

[应用报告] Monitoring of the AKT/mTOR pathway using Internal Standard Triggered-Parallel Reaction Monitoring (IS-PRM)

翻译后修饰研究

磷酸化蛋白质组学

[讲座视频] 蛋白质翻译后修饰研究新进展

[应用报告] 基于二氧化钛富集策略的人类磷酸化蛋白质组大规模鉴定

[应用报告] 内标掺入 SILAC 法用于小量组织的蛋白质组及磷酸化蛋白质组定量

[产品手册] 磷酸化肽段富集相关产品手册

[应用报告] High-pH Reversed-Phase Sample Fractionation for Phosphoproteomic Workflows

[应用报告] Sensitive and Accurate Quantitation of Phosphopeptides Using TMT Isobaric Labeling Technique

[应用报告] Evaluation of search engines for phosphopeptide identification and quantitation

[应用报告] Improved Sample Preparation Workflows for Protein Discovery and Quantification in Pathway Analysis

糖基化蛋白质组学

[评述] Glycoproteomics前沿研究分享

[评述] 最新完整糖肽分析的”铁榔头”—pGlyco

结构生物学研究

交联质谱

[视频讲座] 交联质谱(XL-MS)技术用于功能蛋白质组学研究

[产品手册] 交联质谱产品手册

非变性质谱

[视频讲座] Orbitrap超高分辨非变性质谱技术在抗体药物表征中的最新进展

[应用报告] 使用Q Exactive系列高分辨质谱对ADC与mAb进行变性及非变性条件下分子量测定

氢氘交换质谱

[视频讲座] 赛默飞氢氘交换质谱在蛋白质高级结构解析中的完全解决方案

生物制药色谱质谱表征

[解决方案] 蛋白质药物的液质分析

[应用报告] 蛋白药物质谱应用文集

[应用报告] ADC 单抗药物的分子量、氨基酸序列、糖基化位点以及ADC 药物结合位点鉴定

[应用报告] 单抗药物的分子质量、氨基酸序列以及糖基化位点鉴定

[应用报告] ETD-triggered CID 方法应用于融合蛋白的二硫键分析

[应用报告] DNA & RNA 寡聚核苷酸的准确分子质量测定

[应用报告] Protein Deconvolution 4.0 软件用于单克隆抗体分子量测定

[应用报告] 液相色谱耦联Q Exactive 台式轨道阱质谱仪分析利妥昔单克隆抗体

[应用报告] Characterization of mAb with Microfluidic Chip Electrophoresis Mass Spectrometry

[解决方案] 快速稳健的液相色谱-质谱联用肽图分析全面解决方案

[解决方案] 赛默飞世尔科技生物制药全面解决方案（一）

[解决方案] 赛默飞世尔科技生物制药全面解决方案（二）

[解决方案] 赛默飞世尔科技生物制药全面解决方案（三）

[视频讲座] 赛默飞在生物药研发中的整体解决方案

资源类型

蛋白质组学

不同蛋白提取液对动物组织全蛋白质组鉴定的影响

Increased MS Protein Identification Rates Using 75 cm Long nano LC C18 Separation Columns: Pushing the Limits of Bottom-Up Proteomics

A robust and sensitive capillary flow LC - high-resolution accurate-mass MS platform for discovery and targeted proteomics

An Information System Enabling the Organization and Automatic Annotation of Proteomic Experiments with User-specific Proteins

ASMS 2017 poster 合集

定量蛋白质组学

Q Exactive HF DIA实现高通量血清蛋白质组定量分析

Q Exactive HF DIA实现高通量酵母蛋白质组定量分析

Q Exactive HF DIA实现高通量人蛋白质组定量分析

Q Exactive HF 一小时 DDA 鉴定 DIA 定量 4000 个 Hela 蛋白

精确修饰位点谱图库的建立与磷酸化蛋白质组的 DIA 解析

应用Target MS2 方法对大鼠血浆中人重组干扰素 进行绝对定量的测试方案

Large-Scale Proteome and Phosphoproteome Quantification by Data Independent Acquisition on Ultra-High Field Orbitrap Mass Spectrometers

MS1-Based Quantification Optimization on DIA Methods on a Quadrupole-Orbitrap Mass Spectrometer

Monitoring of the AKT/mTOR pathway using Internal Standard Triggered-Parallel Reaction Monitoring (IS-PRM)

翻译后修饰研究

内标掺入 SILAC 法用于小量组织的蛋白质组及磷酸化蛋白质组定量

基于二氧化钛富集策略的人类磷酸化蛋白质组大规模鉴定

Improved Sample Preparation Workflows for Protein Discovery and Quantification in Pathway Analysis

Sensitive and Accurate Quantitation of Phosphopeptides Using TMT Isobaric Labeling Technique

High-pH Reversed-Phase Sample Fractionation for Phosphoproteomic Workflows

生物制药

使用Q Exactive系列高分辨质谱对ADC与mAb进行变性及非变性条件下分子量测定

ETD-triggered CID 方法应用于融合蛋白的二硫键分析

DNA & RNA 寡聚核苷酸的准确分子质量测定

单抗药物的分子质量、氨基酸序列以及糖基化位点鉴定

Protein Deconvolution 4.0 软件用于单克隆抗体分子量测定

Q Exactive 台式轨道阱质谱仪分析利妥昔单克隆抗体

蛋白药物质谱应用文集

Characterization of mAb with Microfluidic Chip Electrophoresis Mass Spectrometry

[蛋白质组学概述] Mass-spectrometric exploration of proteome structure and function. Nature, 2016, 537, 347. (蛋白质组学经典综述)

[蛋白质组学概述] Universal sample preparation method for proteome analysis. Nature Methods, 2009, 6, 359. (基于超滤管的蛋白质组学样品制备方法)

[蛋白质组学概述] In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nature Protocols, 2007, 1, 2856. (胶内酶解方法)

[蛋白质组学概述] Plasma Proteome Profiling to Assess Human Health and Disease. Cell Systems, 2016, 2, 185. (微量血浆样品制备方法)

[蛋白质组学概述] The One Hour Yeast Proteome. Mol Cell Proteomics, 2014, 13, 339. (一小时酵母蛋白组)

[蛋白质组学概述] A draft map of the human proteome. Nature, 2014, 509, 575. (人类蛋白质组草图)

[蛋白质组学概述] Mass-spectrometry-based draft of the human proteome. Nature, 2014, 509, 582. (人类蛋白质组草图)

[蛋白质组学概述] Electrostatic Axially Harmonic Orbital Trapping: A High-Performance Technique of Mass Analysis. Anal. Chem., 2000, 72, 1156. (Orbitrap质量分析器的原理)

[蛋白质组学概述] Mass spectrometry-based proteomics using Q Exactive, a high-performance benchtop quadrupole Orbitrap mass spectrometer. Molecular & Cellular Proteomics, 2011, 10, M111.011015. (Q Exactive系列仪器原理)

[蛋白质组学概述] Novel Parallelized Quadrupole/Linear Ion Trap/Orbitrap Tribrid Mass Spectrometer Improving Proteome Coverage and Peptide Identification Rates. Anal. Chem., 2013, 85, 11710. (Orbitrap Fusion系列仪器原理)

[定量蛋白质组学] Tandem Mass Tags:  A Novel Quantification Strategy for Comparative Analysis of Complex Protein Mixtures by MS/MS. Anal. Chem., 2003, 75, 1895. (TMT原始论文)

[定量蛋白质组学] Increasing the Multiplexing Capacity of TMTs Using Reporter Ion Isotopologues with Isobaric Masses. Anal. Chem., 2012, 84, 7469. (TMT10原始论文)

[定量蛋白质组学] MultiNotch MS3 Enables Accurate, Sensitive, and Multiplexed Detection of Differential Expression across Cancer Cell Line Proteomes. Anal. Chem., 2014, 86, 7150. (SPS-MS3原始论文)

[定量蛋白质组学] Quantitative temporal viromics: an approach to investigate host-pathogen interaction. Cell, 2014, 157, 1460. (TMT研究宿主-病毒相互作用)

[定量蛋白质组学] A draft map of the mouse pluripotent stem cell spatial proteome. Nat. Commun., 2016, 7, 8992. (TMT用于蛋白在不同细胞器的定位研究)

产品手册

[蛋白翻译后修饰] 磷酸化肽段富集相关产品手册

[定量蛋白质组学] 定量蛋白质组学产品手册

[结构生物学研究] 交联质谱产品手册

[蛋白质组学概述] 肽段脱盐 SPE, spin column, tips

[蛋白质组学概述] 蛋白质组学样品制备和定量产品手册

[蛋白质组学概述] 色谱柱产品手册

[定量蛋白质组学] TMT 产品手册

[蛋白质组学概述] 蛋白质组学常用试剂耗材手册

解决方案

[定量蛋白质组学] 数据非依赖采集(DIA)解决方案

[生物制药] 蛋白质药物的液质分析

[生物制药] 赛默飞世尔科技生物制药全面解决方案（一）

[生物制药] 赛默飞世尔科技生物制药全面解决方案（二）

[生物制药] 赛默飞世尔科技生物制药全面解决方案（三）

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[蛋白翻译后修饰] 最新完整糖肽分析的”铁榔头”—pGlyco

[蛋白翻译后修饰] Glycoproteomics前沿研究分享

[蛋白质组学概述] Hela、Yeast 与 Orbitrap Fusion 的一小时

[定量蛋白质组学] hyperLOPIT给你一双用于细胞器蛋白定位的“火眼金睛” (TMT 用于蛋白的亚细胞定位定量研究)

[定量蛋白质组学] “尾巴”尖上的蛋白组学 (TMT 研究蛋白 N-末端组)

[定量蛋白质组学] 赛默飞推出数据非依赖采集(DIA)解决方案

[定量蛋白质组学] 让“鱼与熊掌兼得”不再是传说--基于Orbitrap Fusion的TMT SPS MS3定量技术 (TMT 高级定量方法简介)

应用报告

蛋白质组学

不同蛋白提取液对动物组织全蛋白质组鉴定的影响

Increased MS Protein Identification Rates Using 75 cm Long nano LC C18 Separation Columns: Pushing the Limits of Bottom-Up Proteomics

A robust and sensitive capillary flow LC - high-resolution accurate-mass MS platform for discovery and targeted proteomics

An Information System Enabling the Organization and Automatic Annotation of Proteomic Experiments with User-specific Proteins

ASMS 2017 poster 合集

定量蛋白质组学

Q Exactive HF DIA实现高通量血清蛋白质组定量分析

Q Exactive HF DIA实现高通量酵母蛋白质组定量分析

Q Exactive HF DIA实现高通量人蛋白质组定量分析

Q Exactive HF 一小时 DDA 鉴定 DIA 定量 4000 个 Hela 蛋白

精确修饰位点谱图库的建立与磷酸化蛋白质组的 DIA 解析

应用Target MS2 方法对大鼠血浆中人重组干扰素 进行绝对定量的测试方案

Large-Scale Proteome and Phosphoproteome Quantification by Data Independent Acquisition on Ultra-High Field Orbitrap Mass Spectrometers

MS1-Based Quantification Optimization on DIA Methods on a Quadrupole-Orbitrap Mass Spectrometer

Monitoring of the AKT/mTOR pathway using Internal Standard Triggered-Parallel Reaction Monitoring (IS-PRM)

翻译后修饰研究

内标掺入 SILAC 法用于小量组织的蛋白质组及磷酸化蛋白质组定量

基于二氧化钛富集策略的人类磷酸化蛋白质组大规模鉴定

Improved Sample Preparation Workflows for Protein Discovery and Quantification in Pathway Analysis

Sensitive and Accurate Quantitation of Phosphopeptides Using TMT Isobaric Labeling Technique

High-pH Reversed-Phase Sample Fractionation for Phosphoproteomic Workflows

生物制药

使用Q Exactive系列高分辨质谱对ADC与mAb进行变性及非变性条件下分子量测定

ETD-triggered CID 方法应用于融合蛋白的二硫键分析

DNA & RNA 寡聚核苷酸的准确分子质量测定

单抗药物的分子质量、氨基酸序列以及糖基化位点鉴定

Protein Deconvolution 4.0 软件用于单克隆抗体分子量测定

Q Exactive 台式轨道阱质谱仪分析利妥昔单克隆抗体

蛋白药物质谱应用文集

Characterization of mAb with Microfluidic Chip Electrophoresis Mass Spectrometry

视频讲座

[生物制药] 快速稳健的液相-质谱联用肽图分析全面解决方案

[生物制药] 赛默飞在生物药研发中的整体解决方案

[蛋白翻译后修饰] 蛋白质翻译后修饰研究新进展

[结构生物学研究] Orbitrap超高分辨非变性质谱技术在抗体药物表征中的最新进展

[结构生物学研究] 交联质谱(XL-MS)技术用于功能蛋白质组学研究

[结构生物学研究] 赛默飞氢氘交换质谱在蛋白质高级结构解析中的完全解决方案

[定量蛋白质组学] Dr. Wilhelm Haas - High-Throughput Multiplexed Quantitative Proteomics and Phosphoproteomics to Understand Cancer Drug Resistance

[定量蛋白质组学] Dr. Noah Dephoure - Multiplexed Quantitative Analysis of PTMs for Monitoring Changes in Protein Interactions and Subcellular Localization

[定量蛋白质组学] Dr. Robert Everley - Multiplexing Targeted Proteomics Assays: Triggered by Offset Multiplexed Accurate mass High resolution Absolute Quantification (TOMAHAQ) on a Lumos Tribrid

[定量蛋白质组学] 定量蛋白质组学的新高度——从shotgun、到DIA、再到target quan完整解决方案

[定量蛋白质组学] Robust Classification of Stem Cell Protein Localization by SPS-MS3.

[蛋白质组学概述] Top-down蛋白质组学用于完整蛋白定性定量

仪器软件方法

Q Exactive 系列仪器中 TMT 定量工作流程的仪器参数优化和 Proteome Discoverer 2.1 软件数据处理方法

Proteome Discoverer 2.1 中文版使用手册

Q-Exactive 静电轨道阱质谱仪简明开关机操作贴士

Orbitrap Fusion (Lumos) 质谱仪的开关机小贴士

LCQ Fleet液相质谱联用仪/LTQ XL液相质谱联用仪简明开关机操作贴士

TSQ液相三重四级杆质谱联用仪 简明开关机操作贴士

TSQ Quantiva和TSQ Endura液质联用仪 简明开关机操作贴士

经典文献

[蛋白质组学概述] Mass-spectrometric exploration of proteome structure and function. Nature, 2016, 537, 347. (蛋白质组学经典综述)

[蛋白质组学概述] Universal sample preparation method for proteome analysis. Nature Methods, 2009, 6, 359. (基于超滤管的蛋白质组学样品制备方法)

[蛋白质组学概述] In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nature Protocols, 2007, 1, 2856. (胶内酶解方法)

[蛋白质组学概述] Plasma Proteome Profiling to Assess Human Health and Disease. Cell Systems, 2016, 2, 185. (微量血浆样品制备方法)

[蛋白质组学概述] The One Hour Yeast Proteome. Mol Cell Proteomics, 2014, 13, 339. (一小时酵母蛋白组)

[蛋白质组学概述] A draft map of the human proteome. Nature, 2014, 509, 575. (人类蛋白质组草图)

[蛋白质组学概述] Mass-spectrometry-based draft of the human proteome. Nature, 2014, 509, 582. (人类蛋白质组草图)

[蛋白质组学概述] Electrostatic Axially Harmonic Orbital Trapping: A High-Performance Technique of Mass Analysis. Anal. Chem., 2000, 72, 1156. (Orbitrap质量分析器的原理)

[蛋白质组学概述] Mass spectrometry-based proteomics using Q Exactive, a high-performance benchtop quadrupole Orbitrap mass spectrometer. Molecular & Cellular Proteomics, 2011, 10, M111.011015. (Q Exactive系列仪器原理)

[蛋白质组学概述] Novel Parallelized Quadrupole/Linear Ion Trap/Orbitrap Tribrid Mass Spectrometer Improving Proteome Coverage and Peptide Identification Rates. Anal. Chem., 2013, 85, 11710. (Orbitrap Fusion系列仪器原理)

[定量蛋白质组学] Tandem Mass Tags:  A Novel Quantification Strategy for Comparative Analysis of Complex Protein Mixtures by MS/MS. Anal. Chem., 2003, 75, 1895. (TMT原始论文)

[定量蛋白质组学] Increasing the Multiplexing Capacity of TMTs Using Reporter Ion Isotopologues with Isobaric Masses. Anal. Chem., 2012, 84, 7469. (TMT10原始论文)

[定量蛋白质组学] MultiNotch MS3 Enables Accurate, Sensitive, and Multiplexed Detection of Differential Expression across Cancer Cell Line Proteomes. Anal. Chem., 2014, 86, 7150. (SPS-MS3原始论文)

[定量蛋白质组学] Quantitative temporal viromics: an approach to investigate host-pathogen interaction. Cell, 2014, 157, 1460. (TMT研究宿主-病毒相互作用)

[定量蛋白质组学] A draft map of the mouse pluripotent stem cell spatial proteome. Nat. Commun., 2016, 7, 8992. (TMT用于蛋白在不同细胞器的定位研究)

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