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Better performance in in vitro transcription using Tris buffered NTPs in comparison to NTPs, titrated with NaOH
RNA transcripts of 100 bases were generated from T7 in vitro transcription reactions using Thermo Scientific NTPs, titrated with NaOH (A) and NTPs, Tris buffered (B). Reactions contained 1 µg of DNA template and were incubated for 2 hours at 37°C. 3 repeats of transcription reaction were performed for each group of NTPs.
