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Invitrogen
HIV1 Reverse Transcriptase Recombinant Protein
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HIV1 Reverse Transcriptase Protein (RP-4912) in WB Ctrl
Western blot analysis of functional recombinant full-length HIV-1 reverse transcriptase (Product # RP-4912). Anti-HIV-1 RT antibody was used at 1:2,000 dilution. A secondary, goat anti-rabbit IgG antibody conjugated with HRP was used at 1:5,000 dilution. An ECL system was used for detection. Protein was run at the following concentrations: 40 ng/ lane, 20 ng/ lane, 4 ng/ lane, and 2 ng/ lane.
产品信息
RP-4912
产品规格
Expression System
E. coli
分类
Recombinant
类型
Protein
偶联物
形式
Liquid
浓度
5000 U/mL
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
产品详细信息
RP-4912 can be used as a positive control in Western blotting with ABR antibodies againt -HIV-1 Reverse Transcriptase, as well as in screening of specific inhibitors for new AIDS treatment .
RP-4912 is composed of two subunits with calculated molecular weights of ~66 kDa and ~51 kDa; see figure 1.
RP-4912 has a specific activity of 5,000 units/mL
靶标信息
HIV-1 reverse transcriptase is an RNA-dependent DNA polymerase of HIV-1(AIDS virus), subtype B origin. It also has RNaseH activity and is an enzyme indispensable to the reproduction of AIDS virus.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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