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产品信息
PHC9481
应用
建议稀释比
已发表文章
产品规格
种属
Human
Expression System
E. coli
氨基酸序列
PLPITPVNAT CAIRHPCHNN LMNQIRSQLA QLNGSANALF ILYYTAQGEP FPNNLDKLCG PNVTDFPPFH ANGTEKAKLV ELYRIVVYLG TSLGNITRDQ KILNPSALSL HSKLNATADI LRGLLSNVLC RLCSKYHVGH VDVTYGPDTS GKDVFQKKKL GCQLLGKYKQ IIAVLAQAF
分子量
21 kDa
分类
Recombinant
类型
Protein
纯度
≥95% by SDS-PAGE
内毒素浓度
<0.1 ng/µg
活性
ED50 ≤ 0.05 ng/mL; determined by the dose-dependent proliferation of human TF-1 cells.
偶联物
形式
Lyophilized
纯化类型
purified
内含物
no preservative
保存条件
4°C
运输条件
Dry ice
产品详细信息
Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile, distilled water or appropriate buffered solution containing 0.1% BSA to regain full activity. Apportion there constituted protein into working aliquots and store at -20°C. Make any further dilutions of the reconstituted protein in buffered solution containing a carrier protein, such as PBS with 0.1% BSA.
Storage: Store the lyophilized protein at 2-8°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles.
靶标信息
Leukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: cholinergic differentiation factor; D factor; differentiation inhibitory activity; differentiation-inducing factor; Differentiation-stimulating factor; hepatocyte-stimulating factor III; human interleukin in DA cells; Leukemia inhibitory factor; LIF; Melanoma-derived LPL inhibitor; MLPLI
基因别名: CDF; DIA; HILDA; LIF; MLPLI
UniProt ID: (Human) P15018
Entrez Gene ID: (Human) 3976
