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Invitrogen
Human POLR2I (aa 63-115) Control Fragment Recombinant Protein
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产品信息
RP-105903
产品规格
种属
Human
Expression System
E. coli
氨基酸序列
DELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCT
标签
His-ABP-tag
分类
Recombinant
类型
Protein
纯度
>80% by SDS-PAGE and Coomassie blue staining
偶联物
形式
Liquid
浓度
≥5.0 mg/mL
纯化类型
purified
保存液
PBS/1M urea, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
产品详细信息
Highest antigen sequence indentity to the following orthologs: Mouse (100%), Rat (100%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-65152. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
靶标信息
POLR2I is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. POLR2I has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests. This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DNA-directed RNA polymerase II 14.5 kDa polypeptide; DNA-directed RNA polymerase II subunit I; DNA-directed RNA polymerase II subunit RPB9; polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa; polymerase (RNA) II subunit I; RNA polymerase II 14.5 kDa subunit; RNA polymerase II subunit B9; RPB14.5
基因别名: hRPB14.5; POLR2I; RPB9
UniProt ID: (Human) P36954
Entrez Gene ID: (Human) 5438
