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产品信息
PV4325
产品规格
种属
Human
Expression System
Insect cells
氨基酸序列
1-299
标签
His-tag
分子量
38.9 kDa
分类
Recombinant
类型
Protein
偶联物
形式
Liquid
浓度
See Label
纯化类型
purified
保存液
50mM tris HCl, pH 7.5, with 50% glycerol, 2mM DTT, 0.02% Triton X-100, 150mM NaCl, 0.5mM EDTA
内含物
no preservative
保存条件
-80° C, Avoid Freeze/Thaw Cycles
运输条件
Dry ice
产品详细信息
For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.
Protein Form: Recombinant, Catalytic
靶标信息
RIPK2 is a serine/threonine kinase that is involved in cell activation, proliferation, differentiation, and apoptosis. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CARD-carrying kinase; CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase; CARD-containing interleukin-1 beta-converting enzyme-associated kinase; EC 2.7.11.1; growth-in; growth-inhibiting gene 30; kinase RIPK2; receptor-interacting protein (RIP)-like interacting caspase-like apoptosis regulatory protein (CLARP) kinase; Receptor-interacting protein 2; receptor-interacting serine-threonine kinase 2; Receptor-interacting serine/threonine-protein kinase 2; RIP 2; RIP-2; RIP-like; RIP-like-interacting CLARP kinase; Tyrosine-protein kinase RIPK2; WUGSC:H_RG437L15.1
基因别名: CARD3; CARDIAK; CCK; GIG30; RICK; RIP2; RIPK2; UNQ277/PRO314/PRO34092
UniProt ID: (Human) O43353
Entrez Gene ID: (Human) 8767
