This peptide corresponds to 17 amino acid peptide near the amino terminus of human UNG2.
PEP-1594 can be used as a blocking peptide with polyclonal antibody PA5-34551. For use as a blocking peptide in Western blot, incubate the peptide with equal volume of antibody for 30 min at 37°C.
The human uracil-DNA glycosylase (UNG) gene encodes both mitochondrial (UNG1) and nuclear (UNG2) forms through differentially regulated promoters and alternative splicing. UNG2 is the major enzyme in the base excision repair pathway that removes uracil residues from DNA that arise through either misincorporation during replication or cytosine deamination. UNG2 can also be bound by the HIV-1 integrase and incorporated into the virion particle, suggesting that it is required to remove uracils from the viral genome. As the intrinsic antiviral protein APOBEC3G generates numerous uracils in the HIV genome during its replication, it may be that the UNG2 contributes to the APOBEC3G-mediated loss of infectivity by generating abasic sites in the viral genome.