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Invitrogen
Mouse IL-2 Recombinant Protein
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Mouse IL-2 Protein (SMIL2) in ELISA Std
Sandwich ELISA analysis of mouse IL-2 was performed using the a Mouse IL-2 Colorimetric ELISA Kit (Product # EMIL2) by loading 50 ul per well of a Mouse IL-2 monoclonal antibody (Product # MM100) in quadruplicate at 0 (blue line), 50% (2.5 ug/ml, red line), and 100% (5 ug/ml, green line) displacement in a plate and then loading 50 ul per well of Mouse IL-2 Recombinant Protein (Product # SMIL2) in dodecuplicate at 850, 170, 34, and 0 pg/ml across a 5 ug/ml Mouse IL-2 monoclonal antibody (Product # MM101) pre-coated plate and incubating for ... View More
产品信息
SMIL2
产品规格
分类
Recombinant
类型
Protein
偶联物
形式
Lyophilized
浓度
0.85 ng/mL
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
产品详细信息
International reference standard is not available. Calibrated to a Thermo reference standard.
Lyophilized product is shipped on cold packs. Stable until expiration if stored desiccated at -20°C. Reconstituted cytokine standards should be stored in aliquots at -70°C. Each aliquot should be used only once and discarded afterwards. Stability of reconstituted and frozen cytokine standards may vary. Controls should be run with each standard curve.
Sufficient For: ELISA standard curve with replicates or as a control in Western blots.
850 pg/ml when reconstituted at the recommend volume
Reconstitute with distilled water at volume indicated on label. Use or freeze product within 1 hour of reconstitution.
靶标信息
Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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