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Invitrogen
Mouse IL-23 (Carrier-Free) Recombinant Protein, eBioscience™
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Mouse IL-23 (Carrier-Free) Protein (34-8231-82) in WB Ctrl
Under non-reducing SDS-PAGE conditions, the heterodimeric cysteine-linked Mouse IL-23 Recombinant Protein Carrier-Free migrates as a 60 kDa protein (Lane NR). Under DTT-reducing conditions it migrates as 43 kDa and 21 kDa polypeptides (Lane R).
产品信息
34-8231-82
产品规格
种属
Mouse
Expression System
Insect cells
氨基酸序列
Mouse IL-23, p40 subunit amino acids Met1-Ser355 (Accession # NM_008352) was coexpressed with subunit p19 amino acids Met1-Ala196 (Accession # AF301619)
分子量
55.6 kDa
分类
Recombinant
类型
Protein
纯度
>98% by SDS-PAGE
内毒素浓度
<0.01 ng/µg
活性
ED50 ≤ 625 pg/mL; determined by the dose-dependent induction of IL-17A expression in mouse splenocytes. This corresponds to a specific actvity greater than or equal to 1.5x10e6 Units/mg.
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
purified
保存液
PBS, pH 6.0
内含物
no preservative
保存条件
-80°C
运输条件
Dry ice
产品详细信息
Description: IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-gamma. Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-gamma production by naive and memory T cells, as compared to IL-12.
Applications Reported: Mouse IL-23 Recombinant Protein Carrier-Free has been reported for use in cytokine bioassays.
Applications Tested: The ED50 of this protein, as measured by IL-17A induction in mouse splenocytes, is less than or equal to 625 pg/mL. This corresponds to a specific activity of greater than 1.5 x 10e6 Units/mg.
Source: Insect cells infected with baculovirus: mouse p40, amino acids met 1-Ser 355, accession # NM_008352 was co-expressed with mouse p19, amino acids met 1-Ala 196, accession # AF301619.
Bioactivity: The ED50 of this protein, as measured by IL-17A induction in mouse splenocytes, is less than or equal to 625 pg/mL. This corresponds to a specific activity of greater than 1.5 x 10e6 Units/mg.
Endotoxin: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE.
Molecular Weight: The heterodimer of p40, amino acids met 23-ser 355, cystine-linked to p19, amino acids leu 20-ala 196, has a predicted molecular mass of 55,616. On non-reducing SDS-PAGE the heterodimeric cystine-linked protein migrates as a 60 kDa protein. The DTT reduced protein migrates as 43 kDa and 21 kDa polypeptides.
Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.
靶标信息
IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-γ. Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-γ production by naive and memory T cells, as compared to IL-12.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: il 23; IL 23 A; Il-12b; Il-12p40; IL-23 subunit alpha; IL-23-A; IL-23p19; IL12B; Il12p40; il23; ILN; Interleukin; interleukin 12B; Interleukin-23 subunit alpha; Interleukin-23 subunit p19; MGC79388; p40; RP23-388G23.1
基因别名: IL-23; Il23a; p19
UniProt ID: (Mouse) Q9EQ14
Entrez Gene ID: (Mouse) 83430
