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Invitrogen
SARS-CoV-2 PLpro (aa 1564-1880) His-tag Recombinant Protein
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产品信息
RP-88305
产品规格
种属
Virus
Expression System
E. coli
氨基酸序列
SARS-CoV-2 Plpro, amino acids Glu1564-Val1880 (Accession # QVD65746.1) with an N-terminal His-tag
标签
His-tag
分子量
36.79 kDa
分类
Recombinant
类型
Protein
纯度
≥90% as determined by SDS-PAGE.
活性
Measured by its ability to cleave a fluorogenic peptide substrate, Arg-Leu-Arg-Gly-Gly-AMC (RLRGGAMC).The Specific Activity is >80pmols/min/ µg
偶联物
形式
Lyophilized
浓度
0.15 mg/mL
纯化类型
purified
保存液
20mM tris, pH 7.4, with 5% mannitol, 500mM NaCl, 5% trehalose, 0.01% Tween 80
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
产品详细信息
It is recommended that the protein be aliquoted for optimal storage.
It is recommended that sterile water (660 µL) be added to the vial to prepare a stock solution of 0.15 mg/mL.
Concentration is measured by UV.
靶标信息
SARS-CoV-2 Plpro is an essential coronavirus enzyme that is required for processing viral polyproteins to generate a functional replicase complex and enable viral spread, further, it is also implicated in cleaving proteinaceous post-translational modifications on host proteins as an evasion mechanism against host antiviral immune responses.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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