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Invitrogen
Basic Mouse MCP-1 ELISA Kit
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产品规格
检测灵敏度
2.2 pg/mL
测定范围
15.6-1,000 pg/mL
检测样本类型/体积
{Supernatant=50 µL, Serum=50 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 10 min
均相（无需洗涤）
No
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
CCL2 (MCP-1)
均相（无需洗涤）
Mouse
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
Kit Overview
The Mouse MCP-1 ELISA kit enables the quantification of Mouse MCP-1 in various biological samples, including human serum, plasma, buffered solutions, and cell culture medium. This assay is specifically designed to exclusively detect both natural and recombinant forms of Mouse MCP-1.
Principle of the assay
The Mouse MCP-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) follows a unique approach to measure the amount of the target molecule. It utilizes a matched pair of antibodies to capture the target and form a sandwich complex. The first antibody is pre-coated on the microplate wells, where samples, standards, or controls are added and bind to the immobilized antibody. The second antibody, acting as the detector, is then introduced, followed by the addition of a substrate solution that generates a measurable signal upon interaction with the enzyme-antibody-target complex. The intensity of this signal directly correlates to the concentration of the target present in the original specimen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : AI323594, Ccl2, HC11, JE, MCAF, MCP-1, MCP1, Scya2, Sigje, SMC-CF
基因ID : (Mouse) 20296
基因符号 : Ccl2
蛋白别名 : C-C motif chemokine 2, H-MCP-1, MGC9434, RP23-350G1.3, MCP-1, Monocyte chemoattractant protein 1, monocyte chemoattractant protein-1, Monocyte chemotactic protein 1, Platelet-derived growth factor-inducible protein JE, small inducible cytokine A2, Small-inducible cytokine A2
UniProt ID (Mouse) P10148
