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Invitrogen
Corticosterone Competitive ELISA Kit
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产品规格
检测灵敏度
18.6 pg/mL
测定范围
78.125-10,000 pg/mL
检测样本类型/体积
{Fecal Extract=50 µL, Plasma=1 µL, Serum=1 µL, Supernatant=50 µL, Urine=2.5 µL}
手工操作时间
1 hr 20 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
7.9%
批内差异
5.2%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
HRP-Conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
HRP-Conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
-20°C
蛋白名称
Corticosterone
均相（无需洗涤）
Chemical
检测试剂盒格式
Competitive ELISA
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Corticosterone ELISA quantitates coritcosterone in serum, plasma, urine, dried fecal extracts or cell culture medium.
原理方法
The Corticosterone Competitive ELISA research-use-only kit is designed to quantitatively measure corticosterone present in serum, plasma, urine, extracted dried fecal samples, and tissue culture media samples. This kit measures total corticosterone in serum and plasma and in extracted fecal samples. A corticosterone stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Please choose the standard range that fits your sample concentrations most appropriately. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A corticosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to corticosterone to each well. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound corticosterone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
CORTICOSTERONE is an adrenocortical steroid that has modest but significant activities as a mineralocorticoid and a glucocorticoid. [National Center for Biotechnology Information. PubChem Compound Database; CID=5753].
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名 : 17-Deoxycortisol, Reichstein's substance H, Kendall's compound B, C21H30O4
