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Invitrogen
Dihydrotestosterone Competitive ELISA Kit
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产品规格
检测灵敏度
23.44 pg/mL
测定范围
39.06-2,500 pg/mL
检测样本类型/体积
{Plasma=100 µL, Serum=100 µL}
手工操作时间
1 hr 20 min
总时间
2 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Micro ELISA Plate (Dismountable)
Reference Standard
Concentrated Biotinylated Detection Ab(100x)
Concentrated HRP Conjugate (100x)
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer(25x)
Substrate Reagent
Stop Solution
Plate Sealer
Product Description
Certificate of Analysis
Reference Standard
Concentrated Biotinylated Detection Ab(100x)
Concentrated HRP Conjugate (100x)
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer(25x)
Substrate Reagent
Stop Solution
Plate Sealer
Product Description
Certificate of Analysis
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
Dihydrotestosterone
均相（无需洗涤）
Chemical
检测试剂盒格式
Competitive ELISA
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
Dihydrotestosterone Competitive ELISA Kit quantifies Dihydrotestosterone in serum and plasma.
The Dihydrotestosterone Competitive ELISA research-use-only kit is designed to quantitatively measure DHT. A DHT standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to DHT is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of DHT in the samples is then determined by comparing the OD of the samples to the standard curve..
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Dihydrotestosterone (DHT) is a biologically active metabolite of the hormone testosterone, formed primarily in the prostate gland, testes, hair follicles, and adrenal glands by the enzyme 5-alpha-reductase by means of reducing the alpha 4, 5 double-bond. Dihydrotestosterone belongs to the class of compounds called androgens, also commonly called androgenic hormones or testoids. DHT is thought to be approximately 30 times more potent than testosterone because of increased affinity to the androgen receptor. [National Center for Biotechnology Information. PubChem Compound Database; CID=10635].
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名 : Andractim, Androlone, Androstanolone, C19H30O2, DHT, STANOLONE
