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Invitrogen
Epiandrosterone ELISA Kit
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产品规格
检测灵敏度
0.126 ng/mL
测定范围
0.098-100 ng/mL
检测样本类型/体积
{Serum=50 µL, Plasma=50 µL, Fecal Extract=50 µL, Urine=12.5 µL, Supernatant=50 µL}
手工操作时间
45 min
总时间
2 hr 30 min
均相（无需洗涤）
No
批间差异
16%
批内差异
8%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Anti-IgG coated 96 well plate
Standard
Assay Buffer Concentrate
Capture Antibody
HRP-conjugated Antigen
Wash Buffer Concentrate
TMB Substrate
Stop Solution
Plate Sealer
Standard
Assay Buffer Concentrate
Capture Antibody
HRP-conjugated Antigen
Wash Buffer Concentrate
TMB Substrate
Stop Solution
Plate Sealer
运输条件
Dry ice
储存
-20°C
蛋白名称
Epiandrosterone
均相（无需洗涤）
Human
检测试剂盒格式
Competitive ELISA
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Epiandrosterone Competitive ELISA quantitates epiandrosterone in serum, plasma, urine, saliva, dried fecal extracts or cell culture medium.|原理方法: The Epiandrosterone Competitive ELISA research-use-only kit is designed to quantitatively measure epiandrosterone independent of species. An epiandrosterone standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with anti-rabbit IgG antibodies. Anti-rabbit epiandrosterone antibodies and epiandrosterone-peroxidase conjugate is added to the wells. The epiandrosterone-peroxidase conjugate and any epiandrosterone in the sample will compete to bind to the anti-rabbit epiandrosterone antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound epiandrosterone-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of epiandrosterone present in the sample.|Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Epiandrosterone is a dehydroepiandrosterone metabolite and a precursor of testosterone and estradiol with hypolipidemic and anabolic property. Epiandrosterone, a potential neurosteroid, appears to bind to the gamma-aminobutyric acid (GABA)/benzodiazepine-receptor complex (GABA-RC), acting as a negative non-competitive modulator of GABA-RC as well as signal through the N-methyl-D-aspartate receptor. In addition this agent inhibits the pentose phosphate pathway (PPP) thereby dilating blood vessels pre-contracted by partial depolarization. Also, epiandrosterone inhibits the synthesis of thromboxane A2 in activated platelets, reduces plasma plasminogen activator inhibitor type 1 and tissue plasminogen activator antigen, increases serum levels of insulin-like growth factor 1 and increases cyclic guanosine monophosphate and nitric oxide synthesis. These effects may improve circulation in the microvasculature. [National Center for Biotechnology Information. PubChem Compound Database; CID=441302].
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名 : 3beta-androsterone, 3Beta-hydroxy-5alpha-androstan-17-one, 5alpha-androstan-3beta-ol-17-one, C19H30O2, Epiandrosterone, Isoandrosterone, Trans-Androsterone
