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Invitrogen
FT4 Competitive ELISA Kit
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产品规格
检测灵敏度
0.94 pg/mL
测定范围
1.56-100 pg/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL}
手工操作时间
1 hr 20 min
总时间
2 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
FT4
蛋白家族
Thyroid hormone
均相（无需洗涤）
All
检测试剂盒格式
Competitive ELISA
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The multispecies Free Thyroxine (fT4) ELISA quantitates fT4 in serum, plasma, and other biological fluids
原理方法:
The Competitive multispecies ELISA research-use-only kit is designed to quantitatively measure Free Thyroxine (fT4) independent of species. A fT4 standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to fT4 added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of fT4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Free Thyroxine (FT4) is a hormone produced by the thyroid gland and plays a crucial role in regulating metabolism, growth, and development in the body. It is transported in the bloodstream and acts on target cells by binding to thyroid hormone receptors. The structure of FT4 is characterized by a tyrosine residue linked to an iodine atom, forming the thyroid hormone. It has a molecular weight of 777.00 g/mol and a chemical formula of C15H11I4NO4. FT4 is primarily produced in the thyroid gland and is regulated by the hypothalamic-pituitary-thyroid axis. Its levels are typically measured in blood tests to diagnose thyroid disorders.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名 : Free Thyroxine
