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Invitrogen
Human Basic TNF alpha ELISA Kit
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产品规格
检测灵敏度
2.3 pg/mL
测定范围
7.8-500 pg/mL
检测样本类型/体积
{Serum=50 µL, Supernatant=50 µL, Plasma=50 µL, Urine=50 µL}
总时间
3 hr 10 min
均相（无需洗涤）
No
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Coated Microwell Strips
Biotin-Conjugate
Streptavidin-HRP
Standard
Assay Buffer Concentrate
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Film
Biotin-Conjugate
Streptavidin-HRP
Standard
Assay Buffer Concentrate
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Film
运输条件
Wet ice
储存
2-8°C
蛋白名称
TNF alpha
蛋白家族
Hormones & Receptors
均相（无需洗涤）
Human
Species (reported)
Human
检测试剂盒格式
Basic ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human Tumor Necrosis Factor Alpha (Hu TNF alpha) ELISA kit offers a precise method for quantifying Hu TNF alpha in human serum, plasma, urine, or cell culture medium. The test will exclusively identify both natural and genetically engineered Hu TNF alpha.
原理方法
The solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) for Human TNF alpha is engineered to quantify the amount of the target captured between a compatible antibody pair. An antibody specific to the target has been pre-coated in the wells of the provided microplate. Samples, standards, or controls are subsequently introduced into these wells and attach to the immobilized (capture) antibody. The sandwich is completed by the addition of the second (detector) antibody. A substrate solution is then added, which reacts with the enzyme-antibody-target complex to yield a measurable signal. The strength of this signal is directly related to the concentration of the target in the original sample.
靶标信息
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
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生物信息学
基因别名 : DIF, TNF, TNF-alpha, TNFA, TNFSF2, TNLG1F
基因ID : (Human) 7124
基因符号 : TNF
蛋白别名 : APC1 protein, Cachectin, DADB-70P7.1, TNF alpha, TNF superfamily, TNF α, TNFα, TNF, macrophage-derived, monocyte-derived, TNF-a, TNF-alpha, Tumor necrosis factor, tumor necrosis factor ligand 1F, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
UniProt ID (Human) P01375
