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Invitrogen
Human CD147/EMMPRIN Rapid ELISA Kit
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产品规格
检测灵敏度
0.47 ng/mL
测定范围
0.78-50 ng/mL
检测样本类型/体积
{Serum=50 µL, Plasma=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
CD147
均相（无需洗涤）
Human
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Human CD147 (Extracellular Matrix Metalloproteinase Inducer) ELISA quantitates CD147 in serum and plasma.
原理方法
The Human Extracellular Matrix Metalloproteinase Inducer (CD147) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Extracellular Matrix Metalloproteinase Inducer (CD147) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Extracellular Matrix Metalloproteinase Inducer (CD147). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Extracellular Matrix Metalloproteinase Inducer (CD147) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
CD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6, EMMPRIN, extracellular matrix metalloproteinase inducer, TCSF, tumour cell-derived collagenase-stimulatory factor) is a 50-60 kDa glycoprotein with multiple glycosylated forms. CD147 is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. CD147 is also a member of the immunoglobulin superfamily. The highest level of CD147 expression is on metabolically active cells, such as lymphoblasts, inflammatory cells, brown adipocytes and malignant tumor cells. CD147 has multiple functions, including facilitating of cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulation of integrin functions, it plays roles in cell development and activation, fetal development or retinal function.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : 5F7, BSG, CD147, EMMPRIN, EMPRIN, HAb18G, OK, TCSF, UNQ6505/PRO21383
基因ID : (Human) 682
基因符号 : BSG
蛋白别名 : 5F7, adhesion molecule, amino acid feature: intracellular domain, aa 707 .. 829, amino acid feature: transmembrane domain, aa 638 .. 706, amino acid feature: extracellular domain, aa 86 .. 637, Basigin, CD147, Collagenase stimulatory factor, EMMPRIN, Extracellular matrix metalloproteinase inducer, HAb18G, Hepatoma-associated antigen, Leukocyte activation antigen M6, OK blood group antigen, retinal glycoprotein, TCSF, Tumor cell-derived collagenase stimulatory factor, unnamed protein product
UniProt ID (Human) P35613
