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Invitrogen
Human CD44var (v5) (Soluble) ELISA Kit
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产品规格
检测灵敏度
0.14 ng/mL
测定范围
0.31-10.0 ng/mL
检测样本类型/体积
{Amniotic Fluid=20 µL, Plasma=20 µL, Serum=20 µL, Supernatant=20 µL, Urine=20 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 10 min
均相（无需洗涤）
No
批间差异
5.8%
批内差异
3.6%
检测仪器
Absorbance Microplate Reader
产品规格
10 x 96 Tests
产品成分
Pre-coated 96 well plates
Standard
Sample Diluent
Assay Buffer concentrate
HRP-conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
HRP-conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
CD44v5
Target site-specificity
Target sub-specificity
Soluble
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human CD44var (v5) ELISA quantitates Hu CD44var (v5) in human serum, plasma, urine, amniotic fluid or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CD44var (v5).
原理方法
The Human CD44var (v5) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
CD44 cell surface antigen is a 100kDa glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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