Search
Invitrogen
Human Cathepsin B Rapid ELISA Kit
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
产品规格
检测灵敏度
0.94 ng/mL
测定范围
1.56-100 ng/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
Cathepsin B
均相（无需洗涤）
Human
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Human CTSB (Cathepsin B) ELISA quantitates CTSB in serum and plasma.
原理方法
The Human Cathepsin B (CTSB) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Cathepsin B (CTSB) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Cathepsin B (CTSB). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Cathepsin B (CTSB) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Cathepsin B is a gene located on chromosome 8p23.1 that encodes a lysosomal cysteine protease involved in intracellular protein degradation. As a member of the cathepsin family, Cathepsin B plays a crucial role in the lysosomal pathway, facilitating the breakdown of proteins into amino acids, which can then be recycled for cellular functions. In addition to its proteolytic activity within lysosomes, Cathepsin B is also implicated in extracellular matrix remodeling, cellular invasion, and apoptotic pathways. Its expression and activity are often upregulated in various cancer types, including breast, colon, and pancreatic cancers, where it contributes to tumor progression and metastasis by degrading extracellular matrix components and promoting tumor cell invasion. Due to these roles, Cathepsin B is considered a potential biomarker for cancer prognosis and a target for therapeutic intervention aimed at inhibiting cancer cell dissemination. Research continues to explore its broader implications in diseases related to protease activity and inflammation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
基因别名 : APPS, CPSB, CTSB, KWE, RECEUP
基因ID : (Human) 1508
基因符号 : CTSB
蛋白别名 : amyloid precursor protein secretase, APP secretase, APPS, Cathepsin B, Cathepsin B1, cysteine protease, epididymis secretory sperm binding protein, keratolytic winter erythema (Oudtshoorn skin disease), lysosomal proteinase cathepsin B, preprocathepsin B, unnamed protein product
UniProt ID (Human) P07858
