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The Human Copeptin (CPP) ELISA quantitates CPP in serum, plasma and other biological fluids
原理方法:
The Competitive Copeptin (CPP) ELISA research-use-only kit is designed to quantitatively measure CPP in Humans. A CPP standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Human CPP is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Human CPP in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Copeptin is a member of vasopressin/oxytocin family. It is a 39-amino acid peptide produced from cleavage of preprovasopressin during precursor processing, and is released along with the hormone arginine vasopressin. Copeptin acts to ensure the proper structural formation of arginine vasopressin during precursor processing.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
基因别名 : ADH, ARVP, AVP, AVP-NPII, AVRP, VP
基因ID : (Human) 551
基因符号 : AVP
蛋白别名 : antidiuretic hormone, AVP-NPII, copeptin, neurohypophyseal, prepro-arginine-vasopressin-neurophysin II, prepro-AVP-NP II, Vasopressin-neurophysin 2-copeptin, vasopressin-neurophysin II-copeptin
UniProt ID (Human) P01185