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Invitrogen
Human Estrogen Receptor alpha ELISA Kit
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产品规格
检测灵敏度
37.5 pg/mL
测定范围
62.5-4,000 pg/mL
检测样本类型/体积
{Cell Lysate=100 µL, Plasma=100 µL, Tissue Homogenate=100 µL, Serum=100 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
Estrogen Receptor alpha
均相（无需洗涤）
Human
Species (reported)
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human Estrogen Receptor Alpha (ERalpha) ELISA quantitates ERalpha in serum, plasma and other biological fluids.
原理方法
The Competitive Estrogen Receptor Alpha (ERa) ELISA research use only kit is designed to quantitatively measure ERa in Humans. An ERa standard is provided to generate a standard curve for the assay and all samples are read off a user generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Human ERa is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Human ERa in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : ER, Era, ESR, ESR1, ESRA, ESTRR, NR3A1
基因ID : (Human) 2099
基因符号 : ESR1
蛋白别名 : DKFZp686N23123, ER36, esr 1, esr-1, hER-alpha36, RP1-130E4.1, ER, ER-alpha, Estradiol receptor, estrogen nuclear receptor alpha, Estrogen receptor, estrogen receptor alpha E1-E2-1-2, estrogen receptor alpha E1-N2-E2-1-2, estrogen receptor alpha splice variant, CTERP-1, ERalphaDup5, ERalphai45a, ERalphai45bL, ERalphai45bS, ERalphai45c, ERalphai56, ERalphai67, Nuclear receptor subfamily 3 group A member 1
UniProt ID (Human) P03372
