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The Human FABP1 (Fatty Acid Binding Protein 1, Liver) ELISA quantitates FABP1 in serum and plasma.
原理方法
The Human Fatty Acid Binding Protein 1, Liver (FABP1) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Fatty Acid Binding Protein 1, Liver (FABP1) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Fatty Acid Binding Protein 1, Liver (FABP1). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Fatty Acid Binding Protein 1, Liver (FABP1) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
FABP1 encodes the fatty acid binding protein found in liver. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABP1 and FABP6 (the ileal fatty acid binding protein) are also able to bind bile acids. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
基因别名 : FABP1, FABPL, L-FABP
基因ID : (Human) 2168
基因符号 : FABP1
蛋白别名 : FABP1, fatty acid binding protein, fatty acid binding protein 1, liver, Fatty acid-binding protein 1, Fatty acid-binding protein, L-FABP, Liver-type fatty acid-binding protein
UniProt ID (Human) P07148