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Invitrogen
Human FABP1 Rapid ELISA Kit
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Product Specifications
Analytical sensitivity
0.28 ng/mL
Assay range
0.47-30 ng/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Hands-on time
15 min
Time-to-result
1 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
FABP1
Species (tested)
Human
Assay kit format
Rapid ELISA Kit
Detector antibody conjugate
HRP
Label or dye
Unconjugated
About This Kit
The Human FABP1 (Fatty Acid Binding Protein 1, Liver) ELISA quantitates FABP1 in serum and plasma.
Principle of the method
The Human Fatty Acid Binding Protein 1, Liver (FABP1) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Fatty Acid Binding Protein 1, Liver (FABP1) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Fatty Acid Binding Protein 1, Liver (FABP1). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Fatty Acid Binding Protein 1, Liver (FABP1) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
FABP1 encodes the fatty acid binding protein found in liver. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABP1 and FABP6 (the ileal fatty acid binding protein) are also able to bind bile acids. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : FABP1, FABPL, L-FABP
Gene ID : (Human) 2168
Gene symbol : FABP1
Protein Aliases : FABP1, fatty acid binding protein, fatty acid binding protein 1, liver, Fatty acid-binding protein 1, Fatty acid-binding protein, L-FABP, Liver-type fatty acid-binding protein
UniProt ID (Human) P07148
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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