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The Human Frataxin ELISA quantitates Frataxin in serum, plasma, and other biological fluids
The Human Frataxin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
FXN is a mitochondrial protein which belongs to the FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA results in Friedreich ataxia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
基因别名 : CyaY, FA, FARR, FRDA, FXN, X25
基因ID : (Human) 2395
基因符号 : FXN
蛋白别名 : Frataxin, mitochondrial, Friedreich ataxia protein, Fxn
UniProt ID (Human) Q16595