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Invitrogen
Human Ghrelin ELISA Kit
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产品规格
检测灵敏度
11.8 pg/mL
测定范围
15.6-1,000.0 pg/mL
检测样本类型/体积
{Plasma=25 µL, Serum=25 µL, Supernatant=25 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 30 min
均相（无需洗涤）
No
批间差异
8.5%
批内差异
6%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Lysis Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Lysis Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
Ghrelin
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human Ghrelin ELISA quantitates Hu Ghrelin in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Ghrelin.
原理方法
The Human Ghrelin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
GHRL encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is an the ligand for the growth hormone secretagogue receptor type 1 and is involved in regulating growth hormone release. Ghrelin has an appeptite-stimulating effect and induces adiposity and stimulates gastric acid secretion. Obestatin may be the ligand for the orphan G protein-coupled receptor GPR39 and is involved in satiety and can have an appetite-reducing effect resulting in decreased food intake.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : GHRL, MTLRP, UNQ524/PRO1066
基因ID : (Human) 51738
基因符号 : GHRL
蛋白别名 : AMCBX1, FIP3, FIP-3, Fip3p, ghrelin 27, ghrelin 28, NEMO, OTTHUMP00000207794, OTTHUMP00000207795, OTTHUMP00000207796, OTTHUMP00000207798, ghrelin/obestatin, IKK-gamma, IP, IP1, IP2, IPD2, Appetite-regulating hormone, ghrelin, growth hormone secretagogue receptor ligand, ghrelin/obestatin preprohormone, Growth hormone secretagogue, Growth hormone-releasing peptide, In2c-preproghrelin, Motilin-related peptide, prepro-appetite regulatory hormone, preproghrelin, Protein M46
UniProt ID (Human) Q9UBU3
