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Invitrogen
Human HSP27 ELISA Kit
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产品规格
检测灵敏度
10.9 pg/mL
测定范围
15.6-1,000 pg/mL
检测样本类型/体积
{Cell Lysate=10 µL, Plasma=10 µL, Serum=10 µL, Supernatant=10 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 30 min
均相（无需洗涤）
No
批间差异
4.3%
批内差异
5.8%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Controls
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Controls
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
HSP27
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human Heat Shock Protein 27(Hu HSP27) ELISA quantitates Hu HSP27 in human serum, plasma, cell lysates, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HSP27.
原理方法
The Human HSP27 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : CMT2F, HEL-S-102, HMN2B, HS.76067, Hsp25, HSP27, HSP28, HSPB1, SRP27
基因ID : (Human) 3315
基因符号 : HSPB1
蛋白别名 : 28 kDa heat shock protein, DKFZp586P1322, Heat shock protein, HS, HSP, Phospho-HSP 27, epididymis secretory protein Li 102, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, heat shock 27kD protein 1, heat shock 27kDa protein 1, Heat shock protein beta-1, Heat shock protein family B member 1, HSP 27, HspB1, SRP27, Stress-responsive protein 27
UniProt ID (Human) P04792
