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Invitrogen
Human ICAM-1 Rapid ELISA Kit
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产品规格
检测灵敏度
0.15 ng/mL
测定范围
0.25-16 ng/mL
检测样本类型/体积
{Serum=50 µL, Plasma=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
ICAM-1 (CD54)
均相（无需洗涤）
Human
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The Human ICAM-1/CD54 (intercellular adhesion molecule 1) ELISA quantitates ICAM-1 in serum, plasma and other biological fluids.
原理方法
The Human intercellular adhesion molecule 1 (ICAM-1/CD54) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of intercellular adhesion molecule 1 (ICAM-1/CD54) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to intercellular adhesion molecule 1 (ICAM-1/CD54). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of intercellular adhesion molecule 1 (ICAM-1/CD54) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : BB2, CD54, ICAM1, P3.58
基因ID : (Human) 3383
基因符号 : ICAM1
蛋白别名 : CD54, cell surface glycoprotein P3.58, epididymis secretory sperm binding protein, Human rhinovirus receptor, Ly 47, MALA2, sCD54, soluble CD54, Surface antigen of activated B cells, ICAM-1, ICAM-1 soluble variant, Intercellular adhesion molecule 1, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, intercellular adhesion molecule-1 precursor, Major group rhinovirus receptor, major group rhinovirus receptor precusor, unnamed protein product
UniProt ID (Human) P05362
