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Invitrogen
Human IGF-1 Rapid ELISA Kit
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产品规格
检测灵敏度
1.01 ng/mL
测定范围
3.12-100 ng/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL}
手工操作时间
15 min
总时间
1 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
IGF1
均相（无需洗涤）
Human
Species (reported)
Human
检测试剂盒格式
Rapid ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human Insulin like Growth Factor 1 (IGF 1) ELISA quantitates IGF 1 in serum and plasma.
原理方法
The Human IGF 1 solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells, where Human IGF 1 in the samples (or standards) binds to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) HRP linked antibody specific for Human IGF 1. Excess conjugate and unbound sample or standard are washed away from the plate. The substrate solution is added to each well, and the enzyme substrate reaction is terminated by the addition of a stop solution, turning the color yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The intensity of this signal is directly proportional to the concentration of Human IGF 1 present in the original specimen, which is determined by comparing the OD of the samples to the standard curve.
Achieve fast and reliable results in just 90 minutes with our Rapid ELISA kit, featuring a streamlined protocol that requires only one wash step.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
IGF1 (Insulin-like growth factor-1) is structurally and functionally related to insulin but has a much higher growth-promoting activity. A variety of cellular responses are induced by IGF1, including cell proliferation, differentiation, migration, and survival. Further, IGF1 is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types in muscle, bone, and cartilage tissue. IGF1 stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regards to enhancing glucose uptake. In circulation, IGFs are predominantly bound to binding proteins (IGFBPs) which prolong the half-life of the IGFs and play a role in delivering them to target tissues. IGF-I is known as one of the most potent activators of the AKT signaling pathway which is known to be a stimulator of proliferation and an inhibitor of programmed cell death. Moreover, mature human IGF-I is 100% homologous with bovine and porcine proteins. Low levels of IGF1 have been linked to Alzheimer's disease. IGF1 is processed from a precursor, bound by a specific receptor, and secreted. Defects in the IGF1 gene are a cause of insulin-like growth factor 1 deficiency and several transcript variants encoding different isoforms have been found.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : IBP1, IGF-1, IGF-I, IGF1, IGFI, MGF
基因ID : (Human) 3479
基因符号 : IGF1
蛋白别名 : H-IGF-1, IGF, IGF1A, IGFIa, IGF-IA, IGF-IB, Insulin like growth factor, M-IGF-1, OTTHUMP00000195084, R-IGF-1, Somatomedin C, IGF-I, Insulin-like growth factor 1, insulin-like growth factor 1 (somatomedin C), Insulin-like growth factor I, insulin-like growth factor IB, Mechano growth factor, MGF, Somatomedin-C
UniProt ID (Human) P05019
