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Invitrogen
Human IL-12 p70 ELISA Kit, High Sensitivity
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产品规格
检测灵敏度
0.1 pg/mL
测定范围
0.16-10.0 pg/ml
检测样本类型/体积
{Plasma=50 µL, Supernatant=50 µL, Serum=50 µL}
手工操作时间
1 hr 20 min
总时间
4 hr
均相（无需洗涤）
No
批间差异
5.4%
批内差异
6.7%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated microwell plate
Biotinylated Detection Antibody
Streptavidin-HRP
Standard
Sample Diluent
Assay Buffer Concentrate
Amplification Reagent I
Amplification Reagent II
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Plate Covers
Biotinylated Detection Antibody
Streptavidin-HRP
Standard
Sample Diluent
Assay Buffer Concentrate
Amplification Reagent I
Amplification Reagent II
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
IL-12 p70
均相（无需洗涤）
Human
检测试剂盒格式
High Sensitivity Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human High Sensitivity Interleukin 12 p70 (HS IL-12 p70) ELISA quantitates Hu HS IL-12 p70 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HS IL-12 p70.
原理方法
The Human HS IL-12 p70 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A special amplification reagent step is added to achieve the high sensitivity capability. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system's response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : CLMF, CLMF2, IL-12A, IL-12B, IL12A, IL12B, IMD28, IMD29, NFSK, NKSF, NKSF1, NKSF2, P35
基因ID : (Human) 3592, (Human) 3593
基因符号 : IL12A, IL12B
蛋白别名 : CLMF p35, CLMF p40, cytotoxic lymphocyte maturation factor 1, p35, 20C2, il 12, IL 12p70, IL-12 p75, IL12A/B, IL12AB, ILN, Interleukin, Interleukin-12 p70, MGC151228, MGC151232, Cytotoxic lymphocyte maturation factor 35 kDa subunit, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p35, IL-12 subunit p40, IL-12, subunit p35, IL-12A, IL-12B, IL12, subunit p40, IL35 subunit, interleukin 12, p40, interleukin 12A (natural killer cell stimulatory factor 1, p35), interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40), interleukin-12 alpha chain, interleukin-12 beta chain, Interleukin-12 subunit alpha, Interleukin-12 subunit beta, natural killer cell stimulatory factor, 40 kD subunit, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NK cell stimulatory factor chain 2, NKSF1, NKSF2
UniProt ID (Human) P29459, (Human) P29460
