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Invitrogen
Human IRS1 (Phospho) [pS312] ELISA Kit
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![Human IRS1 (Phospho) [pS312] ELISA Kit](https://www.thermofisher.cn/antibody/images/650/4487.jpg?time=20240507 "Human IRS1 (Phospho) [pS312] ELISA Kit")
![Human IRS1 (Phospho) [pS312] ELISA Kit](https://www.thermofisher.cn/antibody/images/150/4487.jpg?time=20240507)
![Human IRS1 (Phospho) [pS312] ELISA Kit](https://www.thermofisher.cn/antibody/images/150/KHO0521_SC_080326.jpg?time=20240507)
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产品规格
检测灵敏度
<1 U/mL
测定范围
1.6-100 U/mL
检测样本类型/体积
{Cell Lysate=10 µL}
手工操作时间
1 hr 20 min
总时间
4 hr
均相（无需洗涤）
No
批间差异
7.4%
批内差异
5.8%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Detection Antibody
anti-rabbit-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Standard Dilution Buffer
Detection Antibody
anti-rabbit-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
IRS1
Target site-specificity
Phosphorylated Ser312
蛋白亚型
Adaptor Proteins
蛋白家族
Other Proteins
均相（无需洗涤）
Rat, Human, Mouse
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human IRS1 (Phospho) [pS312] ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) designed to detect and quantify the level of IRS1 (Phospho) [pS312] in fresh or frozen human cell lysates. Cross-reactivity has been observed in mouse and rat cells. The assay recognizes both natural and recombinant IRS1 (Phospho) [pS312].
原理方法
A monoclonal capture antibody specific for IRS1 (Phospho) [pS312] has been coated onto the wells of the 96-well plate. During the first incubation, standards of known content and unknown samples are pipetted into the wells and the antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for the target protein is added to the wells and serves as a detection antibody by binding to the immobilized protein captured during the first incubation. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of target protein present in the original specimen and the optical density can be read on a standard microplate reader.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : G972R, HIRS-1, IRS-1, IRS1, IRS1IRM
基因ID : (Human) 3667, (Rat) 25467, (Mouse) 16367
基因符号 : IRS1, Irs1, Irs1
蛋白别名 : Insulin receptor substrate 1, IRS-1, pp185
UniProt ID (Human) P35568, (Rat) P35570, (Mouse) P35569
